| OBJECTIVE In order to discuss the application and perspective of NGF in curing Alzheimer's disease and other neurodegenerative diseases, we study the effect and mechanism of NGF from the Venom of Vipera russelli Siamensis Smith of AD mice induced by soluble A(325-35.METHODS Separate mice which has been lateral buried into some groups: sham group, AD model group, positive drug control group(Piracetam,0.02mg·μl-1),NGF group 1(0.2μg·μl-1),NGF group 2(0.28μg·μl-1),NGF group 3(0.4μg·μl-1),NGF group 4(0.56μg·μl-1),and NGF group 5(0.8μg·μl-1).We also set up control group.Each group has ten mice. We use morris water maze and step-down test to study the effect and mechanism of NGF from the Venom of Vipera russelli Siamensis Smith of AD mice induced by soluble Aβ25-35.We study the effect and its possible mechanism of NGF from the Venom of Vipera russelli Siamensis Smith through the evaluation of the activity of acetyl cholinesterase (AchE), the activity of choline acetyltransferase (ChAT) and the content of acetylcholine (Ach) in the brain of AD mice, HE and Nissel dyeing, and the activity of SOD, MDA, GSH AND T-AOC.Also,we use Piracetam as positive drug.RESULTS 1.The result of the effect of NGF of learning and memory of AD mice shows that:accompanied by increasing of training days, the latency (mice spend to find the platform under water) of all groups of mice (except for the highest dose group of NGF) shorten little by little. In the last day test, there is no significant difference between the number of crossing platform and the time spent in target quadrant of sham group and control group.At the same time, there is statistically significant difference between AD and control group (P<0.05). Still,related to the number of crossing platform and the time spent in target quadrant, there is no significant difference between NGF group 1 or 2 and AD mice, but, there is statistically significant difference between NGF group 3 or 4 and AD mice (P<0.0 or P<0.05).In the step-down test, there is statistically significant difference between AD mice and control group on the latency of sixth day (jump down the platform) and the wrong frequency within three minutes (jump down the platform) (P<0.01).There is no significant difference between sham and control group (P<0.01).Moreover, there is statistically significant difference between NGF 1,2,3,4 and AD group (P<0.01 or P<0.05).2.Discuss the mechanism and effect of NGF on learning and memory abilities of AD mice:from HE and Nissl staining, nerve cells of hippocampi have significant difference between AD mice and control group, such as that cell line is not clear anymore, cell spray sparse and the number of normal cells decrease while there is no change between sham and control group.Moreover, there is significant difference between NGF group 4 and AD mice, for an instance, the cell line is clearer, cell spray is in better order and the number of normal cells is bigger. There is no significant difference between sham and control group on the activity of AchE, Ach, ChAT, SOD, MDA, GSH, T-AOC (P>0.05) while there is statistically significant difference between AD and control group.The changes of all items depend on dose changes among all NGF groups (increase or decrease). Related to the changes of activity of Ach, SOD,GSH, there is significant difference between NGF group 2 and AD group (P<0.05).Related to the change of all items,there is significantly changes between NGF group 3 or 4 and AD group (P<0.05 or P<0.01).Related to the changes of activity of Ach, SOD,there is significant difference between NGF group 5 and AD mice.CONCLUSION NGF from the Venom of Vipera russelli Siamensis Smith can improve some ability of AD mice induced by soluble Aβ25-35 such as the learning and memory activity and pathological damage, the change of cholinergic system and the function of oxidation resistance. |
PDF Full Text Request