Variabilities Of Serum Proteomic Spectra In Patients With Non-hodgkin Lymphoma

Objective:①To develop a diagnosis model of serum proteins mass spectra for NHL and evaluate its clinically applied value.②To to investigate the serum proteins mass spectra among different pathologic patterns.③To to investigate the differential expression of serum proteins mass spectra among different clinical features in patients of diffuse large B cell lymphoma(DLBCL) selected from this data.④To investigate the changes of serum proteins mass spectra after chemotherapy.Methods:①SELDI technology and CM10 protein chip were used to analyze mass spectra of serum samples from 116 controls and 109 NHL patients , Biomarker Wizard and Biomarker Pattern Software(BPS) were used to analyze the data and establish diagnosis model,with which a blind test was conducted in another 25 healthy controls anf 21 NHL patients.②Several subtypes of histopathology were analyzed by SELDI technology and CM10 protein chip.③SELDI technology and CM10 protein chip were used to analyze mass spectra between stages,IPI scores, levels ofβ2-MG and LDH in 52 patients of DLBCL.④The changes of mass spectra was analyzed after chemotherapy in 21 patients by Self-paired T test.Results:①41 protein peaks were significantly different between healthy controls and NHL patients,mainly including these paeks (M2728,M2754,M3413,M4099,M4194,M4367,M5642,M5924,M6451,M8726,M8793,M6645). by Biomarker Wizard and Biomarker Pattern ,a primary diagnosis model of NHL was set up consisting of 2 protein peaks with M/Z values of 4367Da,2728Da , bind test confirmed a sensitivity of 95.27%,specificity of 100%.②Three protein peaks(M8575,M6438,M2728) were up-regulated in B cell lymphoma,while were down -regulated in T cell lymphoma; three protein peaks(M8575,M8703,M6633)were up-regulated in indolent B cell lymphoma, while were down -regulated in DLBCL;two protein peaks(M2728,M3812) were up-regulated in mantle cell lymphoma,while were down -regulated in DLBCL; no significantly different peaks were found between activated B cell lymphoma and indolent,between NK-T cell lymphoma and non NK-T.③In DLBCL,one protein peak(M2728) was up-regulated in stageⅠ-Ⅱ,while was down-regulated in stageⅢ-Ⅳ; two protein peaks(M2728,M4085) were up-regulated in scores of IPI 0-1,while were down -regulated in scores≥2; three protein peaks(M3261,M4085,M5636)were up-regulated in normal levels ofβ2-MG, while were down -regulated in abnormal;no significantly different peaks were found between normal levels of LDH and abnormal.④No changes of protein peaks were found in 21 patients after chemotherapy.Conclusions:①The results suggest that SELDI-TOF-MS serum protein profiles could differentiat NHL petients from healthy controls with a high sensitivity and specificity.②Different protein peaks were found in several pathologic patterns.③Among DLBCL, Different protein peaks were found between stages,IPI scores, levels ofβ2-MG,and were not between levels of LDH.④No changes of protein peaks were found in 21 patients after chemotherapy.