Islet Cells And Bone Marrow Mesenchymal Stem Cells Transplantation For Treatment Of Diabetic Rats

Objective:To explore the protection of bone marrow-derived mesenchymal stem cells in the islet transplantation in the potential of islet graft.Methods:Select the four-week-old SD rats as donor, Density gradient centrifugation mesenchymal stem cells, Separation of collagenase digestion and islet cells, Preparation of STZ-diabetic model of SD rats, will become the model rats were divided into:A group (islet cell and bone marrow mesenchymal stem cell transplantation group) 10, B group (pure islet cell transplantation group) 10, only, are injected through the portal vein into the liver channel, Another set C group (control group) 5, only through the portal vein injection of normal saline.The use of density gradient centrifugation mesenchymal stem cells,Rat islet cell isolation and culture, the use of islet cells isolated by collagenase digestionDiabetic mouse model, SD rats, after overnight fasting intraperitoneal injection of 2% Streptozotocin 60mg/kg, a row of more than two times blood glucose 16.5mmol/L and the stability of 2-3 days making tips modeling successMesenchymal stem cells and islet cell transplantation, as well as a separate islet cell transplantation, and compared each group to improve the situation in rat blood glucose, insulin secretion, as well as morphological changes of pancreatic tissue.Results:1). morphological characteristics of MSCs in vitro by density gradient centrifugation of MSCs relatively uniform shape. Gang inoculation was suspended growth, the next day most of the already adherent morphology with round-based, showing irregular, triangular, polygonal and other shapes. As the incubation time to extend the suspension of the round cells decreased, adherent cells began to divide the proliferation of spindle-shaped, spindle-shaped, Nest-like growth 7 days after cell fusion into a single layer, into the whirlpool-like arrangement. Reached after 2,3-generation cell morphology becomes uniform, may have scattered off the growth of the growth can also be set.2). islet cell morphology after collagenase digestion of rat pancreatic tissue of the suspension was placed under the inverted microscope observation, see the different sizes, round or oval cell mass of light brown, and scattered cells with double-hydrazone sulfur dyed red or scarlet typical islet cell clusters, observed under the microscope, islet diameter sizes, ranging from the number of islet cells, exocrine organization did not coloring.3). was successfully prepared diabetic rats, SD rats by intraperitoneal injection 2% Streptozotocin 60mg/kg, a continuous blood glucose in excess of two sub-16.5mmol/L and to stabilize the 3 days.4). Three groups after transplantation, respectively changes in rats with blood glucose monitoring, statistics of the three time points of data and conduct analysis of variance results P<0.05, statistically significant, in which islet co-transplantation group compared transplantation group alone there are significant difference in the transplantation group and the pure islet cell transplantation group, blood glucose levels can be maintained at the normal level; combined transplantation group, the level of insulin secretion than the other two groups are also significant differences.Conclusions:Bone marrow-derived mesenchymal stem cells protect islet grafts to extend the islet cell graft survival time, MSCs in vitro expansion is relatively easy, and rich sources for islet transplantation to open up a new way.