| BackgroundDiabetes mellitus (DM), a common and frequently- occurring disease, leading to various complications is an important cause of death in diabetes patients. Diabetic cardiomyopathy(DCM) is one of the most serious complications of diabetes, which is the primary injury of diabetic patients with myocardial widespread structural abnormalities, ultimately leading to left ventricular hypertrophy, diastolic and/or systolic dysfunction in the disease, the symptoms including chest tightness, shortness of breath, palpitation, heavy-palpitation, Chest Pain, cardialgia. As modern medicine is concerned, focusing on symptoms is the only one method for DCM. Compared with modern medicine, holistic modulation in TCM to prevent complications of DM appears predominance, which can take good effects on curing symptoms and roots. In sum, it is great significant to search a safe and effective method in TCM, aiming to treat DM and its complications.Our preliminary research showed buckwheat's total flavanone has the function of reducing blood sugar, lowering blood fat, improving sugar tolerance, oxidation resistance, eliminating free radical and so on. On the basis of the form experiment, the research adds the buckwheat with rhizoma polygonatum (Huangjing), rehmanniae radix (Shengdi) and so on to composing some formulae, namely Qiaojing Compound Preparation (QJCP). The rat model of DCM was established by means of high-fat diet together with injection of streptozocim(STZ). Trying to illustrate effects of QJCP on DM and DCM, objective indexes are observed, so as to observe QJCP intervention to experimental DCM and its possible mechanism.Objective:The rat model of DCM was established by high-fat diet together with injection of streptozocim (STZ), to investigate the effects of QJCP on experimental DCM and its possible mechanism.Method:1. Animal model establishment: Healthy male SD rats(♂), 200±20g body weight, adaptability keeping fifty days after 12 randomly selected as the blank control group, ordinary food be given. The rest rats as a high-fat and lipid emulsion gavage once daily, then the rats with injection STZ 30 mg/kg and repeat injection to form model in 7 days. According to the criteria as the following : fasting blood glucose value≥11.1mmol/L and the rats were performed glucose i.g. 2g/Kg,the blood glucose was examined after 120 minutes and the successful model is the glucose value was higher than FBG by 20%.2. Animal groups and drug delivery program: type2 DM was induced in rats by injection of STZ. The rats with type2 DM were randomly divided into four groups: model control group, QJCP (high and low)-treated group, and Xiaokewan-treated group. Meanwhile, QJCP (high and low) and Xiaokewan were administrated by ig. at the dose of 1.2g?kg-1, 0.6g?kg-1 and 0.83g?kg-1. Then, rats in control and model group were accordingly given distilled water and ordinary food. Rats in each group were infused with the drugs once a day for eight weeks respectively.3. Indexes①The general condition of rats (coat color, spirit, drinking water volume, urinary volume and so on)②fasting blood glucose(FBG)③total cholesterol(T-CHO), triglyeride(TG),free fatty aids(FFA)④Serum levels of tumor necrosis factor-α(TNF-α)⑤Heart weight index (HWI)⑥HE-stain, to observe pathological changes in myocardium under microscope⑦Immunohistochemical techniques were used to detect TNF-αexpression. ⑧Use the electron microscope to observe the renal tissue.Result:1. General condition: the rats in the control group are characterized by poor spirit, keeping still, coat color grey, urinary volume and drink water volume increasing sharply. Rats in other drups-groups have improvements in every indexes.2. Effects of QJCP on FBG in ratsFBG of rats in controlled group is much higher than that in blank group(P<0.01), compared with FBG in model group, FBG in every drug- groups is lowed down dramatically(p<0.01).3. Effects of QJCP on blood fat in ratsCompared with normal group, T-CHO, TG and FFA of rats increase sharply in model group(P<0.01). These indexes low down significantly in QJCP group (P<0.01).4. Effects of QJCP on TNF-αlevel in rats'serumTNF-αlevel in model group is much higher that n normal group (P<0.01), compared with that in model group, TNF-αin QJCP group decreases sharply, among which QJCPH has the best effects (P<0.01).5. Effects of QJCP on HWI indexThere is a significant difference between model group and normal group in HWI (P<0.01). HWI reduces fairly in QJCP group, among which QJCP H has the best effects (P<0.01).6. Effects of QJCP on myocardium pathological changesUnder microscope, there can be seen that myocardium fibers arrange disorderly, cells loose and large, intercellular space loosened, fibers broken down, infiltration of inflammatory corpuscle emerge. There is significant improvement in pathological changes in myocardium, especially in QJCPH group.7. Effects of QJCP on expression of TNF-αin rats myocardiumResults of immunol histochemistry indicating: Eepression of TNF-αget high in rats from model group, brownish-yellow staining emerge significantly in myocardium. However, that of QJCPH gets low, few of brownish-yellow staining.8. Effects of QJCP on ultrastructure change in rats myocardium There are focal lysis, zona pellucida widened, myoseptum abnormal, mitochondria dropsy, hogback in mitochondria arranging sparse or disappearing in rats of model group. Compared with that in model group, ultrastructure change is lighter in QJCPH group.Conclusion:The QJCP can inhibit the pathological changes of diabetic cardiomyopathy.The mechanism may be related with inhibition of FBG,CHO,TG,FFA and TNF-αlevel. |
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