Barbed Formononetin Regulate Tau Protein In Hippocampus Of Ovariectomized Rats

Aims: Study of formononetin hyperlipemia rats to ovarian tissue in the hippocampus tau protein adjustment, to investigate the senile dementia and hyperlipidemia.Methods: The 40 successfully ovariectomized wistar rats were randomly divided into four: the model group, Nilestriol group, low dose of thorns formononetin group, high dose of thorns formononetin group, 10 in each group. Another 10 wistar rats were randomly selected for the sham group.JiaShouShu group were opened abdominal, small fat around the ovary were removed after the ball off abdomen;The model group, and group etidronic formononetin low dose group and high dose group were executed bilateral oophorectomy. Vaginal exfoliated cells that wash the success was castrated. And on the first 11 days each feeding with high fat prandial, lasting 8 weeks. Blood test TC, TG, LDL-C, HDL-C, decapitated bilateral hippocampus store at-80℃.In western blot and realtime - PCR detection each rats had hippocampal tau protein expression and the level of gene.Results: 1 The model group the hippocampus tau protein expression than JiaShouShu group of (0.3888±0.0361 Vs 0.2389±0.0352,P<0.05), Positive control (group) etidronic model group (0.2317±0.0324 Vs 0.3888±0.0361, P < 0.05), Spurs formononetin low dose group was significantly lower than the model group (0.0295±0.2401Vs0.3888±0.0361, P < 0.05), hile spurs formononetin high-dosage groups are model group was significantly lower (0.0328±0.2273 Vs0.3888±0.0361 P < 0.05) and is obviously superior to reduce positive control (0.2273±0.0328/0.3888±0.0361 Vs0.2317±0.0324/0.3888±0.0361, P > 0.05). 2 The model group the hippocampus tau mRNA expression is sham group were significantly higher3.76(2.87-4.92), Positive control (group) etidronic model group was significantly lower than(0.81(0.69-2.22)Vs3.76(2.87-4.92),P<0.05), Spurs formononetin low dose group was significantly lower than the model group(2.57(2.48-2.66) Vs 3.76(2.87-4.92),P<0.05) thorn formononetin high-dosage groups in model group was significantly lower (1.68(1.27-2.23)Vs3.76(2.87-4.92),P<0.05), but the expression is tau RNA positive control did not show obvious advantage.3 Model rats within the hippocampus is the expression JiaShouShu group Era 3.97 (3.16 - reducing The Times, 499) with statistical significance, Neil female rat hippocampal alcohol group within the expression is an Era of increased 5.94 (9.94-7.94, its differences) with statistical significance, Spurs formononetin low dose group within the hippocampus Era in model group increased expression of 124 (114-1.75), the differences as a statistically significant, Spurs formononetin high-dosage groups within the rat hippocampal Era in model group expressed higher 2.95 (2.10), its dope-containing - as a statistically significant differences, Model rats within the hippocampus is the expression JiaShouShu Er beta 5.03 reduced group (3.48-7.26), its differences as a statistically significant, Neil female rat hippocampal alcohol group within the expression of a model of Er beta 16.56 increased 12.38 (group), the 22.16 - as a statistically significant differences, Spurs formononetin low dose group within the hippocampus Era in model group expressed higher 1.71-1.88 (twice), and the difference between 2.07] are statistically significant, Spurs formononetin high-dosage groups within the rat hippocampal Era in model group expressed 3.55 (701 - increased 499) as a statistically significant differences.4 Pearson relevant examination five rats within the organization taumRNA hippocampal ERa and the mRNA level, both positively related (r = 0.889, P < 0.05). Pearson relevant examination five rats within the organization taumRNA and ER hippocampus, both the beta mRNA level negatively correlated (r = - 0.758, P < 0.05). Above the correlation analysis can preliminary explanation: to stab formononetin tau protein impact is likely to be through adjusting the level and Er beta role, but its specific combination way and biological effect remains to further study.Conclusion: Castrated hyperlipidemia model can make the hippocampus tau protein pression anomalies,suggesting that estrogen level with a high-fat diet can make rats neurological unusual,to a certain extent, reflecting neurodegenerative risk factors. Spurs formononetin to the hippocampus tau protein adjustment function, in some extent could tip thorn formononetin to hyperlipidemia and neurodegenerative to a certain extent.