Changes Of Tumor Necrosis Factor-α, Interleukin-1,-6 And -8 In FFulminant Acute Pancreatitis Of Rats

Background:In recent years,literatures report that the progress of disease in a fewpatients of severe acute pancreatitis(SAP) is rapid.Mutiple organ dysfunctionsyndrome(MODS) appears in early stage.And it could not block the progress ofdisease by positively nonoperative treatment.The mortality is also very high.Itis called FAP. In AP,some inflammatory cells and acinar cells of pancreas releasecytokines and mediators of inflammation. they cause inflammatory reaction whichlead to SIRS and MODS.Present researchs show that inflammatory mediators likecytokines play important roles in MODS and SAP.But the changes of cytokines infulminant acute pancreatitis(FAP) is not found in sino-foreign literatures we lookup to.The roles of cytokines in FAP are not clear also.So the research of the changesof cytokines in FAP may reveal the characteristics of FAP compared with acutepancreatitis(AP).Objective:To determine the concents of tumor necrosis factor-α(TNF-α),interleukin-1(IL-1),interleukin-6 (IL-6) and interleukin-8 (IL-8) in serum in rat models ofFAP,and explore the role of them in pathogenesis of FAP.Methods:FAP models in Wistar male rats were established by intraperitoneal injectionand subcutaneous injections of 20%L-Arg of 120 mg/100g body weight.the changesof TNF-α,IL-1,IL-6 and IL-8 in plasma were determined.Meanwhile,PM and dry andwet ratio were analyzed.Results:1,On PM and dry and weight ratio,there were significant differences(P＜0.01)between two groups starting from 6h(6,12,24,48,72,120h).PM reached the peakat 48h and the secondary peak at 24h.Dry and wet ratio decreased to nadir at 48h. 2,Serum IL-1 increased and peaked at 6 hours,then decreased to normal levelgradually.3,the level of serum TNF-αwas bimodal pattern.It reached the first peak at12 hours and the secondary peak at 120h.4,IL-6 and IL-8 both increased and peaked at 24 hours,then both of them decreasedto normal level gradually.Conclution:IL-1 is initiator of FAP.It promotes growth of TNF-α, which then stimulatesthe level of IL-6 and IL-8 to increase to the peak. IL-6 and IL-8 are congruentwith the severity of the pathological changes of pancreatic tissue.