The Study On The Development And Morphology Of Trigeminal Ganglion Cells In Human Fetus

Objective: To observe morphology and structure of trigeminal ganglion cells and the development of trigeminal ganglion cells with the age increased in human fetus. Methods: Taking different age fetuses perfused after fixation; removed trigeminal ganglion under surgical microscope, conventional HE stained, Fetus trigeminal ganglion were observed by light microscopy, cells counted, and image analysis instrument measured the area, perimeter and diameter of trigeminal ganglion cells. Observed the intracellular ultrastructure by electron microscopy and compared organelles changes between the different age period; DiI dyeing crystals was embedded into the trigeminal nerve root of the specimen. It was placed at 37℃thermostatic box for 3 months and waited for the diffusion of DiI dyeing crystal;another one was used to do frozen Nissl stained Results: As the age increased, the number of trigeminal ganglion cells had no significant changes,but grown in diameter, the area and perimeter of cells increased significantly. During the 18 to 20 weeks development, the neurons in the trigeminal ganglion cells tightly packed, with little cytoplasm and can observe a small number of mitochondria which is barely seen the cristae, there were few other organelles,25 weeks, mitochondria cristae became longer,rough endoplasmic reticulum shape appeared, some showed a longitudinal tubules, the mature Golgi complex appeared at 27 weeks old fetus,. After 32 weeks, the development of mitochondria, rough endoplasmic reticulum and other organelles approached maturity; the lysosome can be seen under electron microscope until 33 weeks; At 36 weeks, the structure and function of all kinds of intracellular organelles basically have completely developed; trigeminal cells can be distinctly labeled by DiI. Conclusion: The morphology development of trigeminal ganglion cells in human fetus matured at 32 weeks (8 months);The structure and function gradually improved with gestational age increased during human fetal trigeminal ganglion cells development,32 to 36 weeks (8～9 months) was an important period of cells differentiation and development...