| Objective:An intestinal ischemia-reperfusion (II/R) young rat model was established, the lidocaine injection was used to intervene for liver injury in the model. The aim of this study was to observe histological damage of the liver tissue, detect the values of ALT in the young rats serum and the expression of MDA, MPO, ICMA-1, TNF-α(tumor necrosis factor-a) in the liver tissue, which explore the protective effect of lidocaine injection in young rats with intestinal ischemia-reperfusion injury in liver and paving the way for clinical experiment and guidance in future.Methods:80 Sprague Dawley (SD) rats at 4 weeks old were divided into four groups:(1) sham-operation group (10 rats), (2) intestinal ischemia group (I group, 10 rats). (3) ischemia-reperfusion group (II/R group,30 rats), (4) lidocaine pretreatment group (Lid group,30 rats). The sham-operation group only exposure to the superior mesenteric artery (SMA) did not other treatments,60 minutes (min) after death. Ischemia group exposure and occlusion of the SMA,60min after the animals were killed. Ischemia-reperfusion group, clipped the SMA 60min, injected 0.5ml physiologic saline as control before reperfusion in the femoral vein. Lidocaine pretreatment group clamped the SMA 60min. injected lidocaine 2mg/kg (diluted in 0.5ml of physiologic saline) before reperfusion in the femoral vein. Ischemia-reperfusion group and the lidocaine group were reperfusion 30min, 60min.120min,10 animals were sacrificed at each time point. After the animals were sacrificed, taked the blood serum and detected values of alanine aminotransferase (ALT), the liver MDA content and MPO activity were detected by spectrophotometer. The expression of ICAM-1'values in liver tissue were measured by enzyme-linked immunosorbent assay (ELISA). The liver tissue used H-E staining for pathological examination, the liver expression of TNF-a were detected by immunohistochemistry. Homogeneity of variance based on the information, the data comparison group used One-factor analysis of variance (One-Way ANOVA test), LSD-t test or Kruskal-Wallis test. When Kruskal-Wallis test'results was statistically significant, group comparison used Dunnett T3 test. Take a=0.05 level test. P<0.05 indicated statistical significance.Results: 1. General H-E staining showed that:Sham-operated group showed normal liver. Intestinal ischemia group of liver tissue shows mild hyperemia and edema. Intestinal ischemia-reperfusion group liver tissue congestion and inflammatory cell infiltration, liver cells showed edema, the cytoplasm of osteoporosis, with the extent of reperfusion time, the damage gradually increased. The liver tissue injury of lidocaine pretreatment had the same trend of the injury in intestinal ischemia-reperfusion model, lighter than intestinal ischemia-reperfusion group, but the liver tissue injury of lidocaine pretreatment group 30min and 60min were same with intestinal ischemia group, the liver tissue injury of lidocaine pretreatment 120min was severer than intestinal ischemia group.2. Measured serum ALT values, malondialdehyde content, myeloperoxidase content, intercellular adhesion molecule-1 value and tumor necrosis factor-a value in young rats Intestinal ischemia-reperfusion group, the results showed that:(1) The values of all targets in intestinal ischemia group were higher than that in sham-operation group, there were significant different statisticly compared between two groups (P<0.01).(2) Intestinal ischemia-reperfusion group values increased from reperfusion 30min, in addition to ALT values reperfusion 60min increased peak and gradually decreased after the peak, the other values still going up until reperfusion 120 minutes.(3) Intestinal ischemia-reperfusion group at each time point values were higher than sham-operation, ischemia group and Lidocaine pretreatment group, after compared the results showed significantly (P<0.01). (4) Intestinal ischemia-reperfusion group compared, in addition to ALT II/R30min compared with II/R 120min was no significant difference (P>0.05), the other results were significantly different (P<0.01).2. After the intervention of lidocaine in young rats, liver tissue and blood test results of each indicator:(1) Lidocaine pretreatment group values increased from reperfusion 30min, in addition to the ALT values to reperfusion 120min had been on the rise, the other values peaked to reperfusion 60min, and then gradually decreased.(2) Lidocaine pretreatment group at each time point values were higher than sham-operation, the results had significantly different compared between two groups(P<0.01).(3) Lidocaine pretreatment group compared with ischemia group, ALT and TNF-a values were lower than ischemia group, group compared, results were significantly different(P<0.01); Lid60min and Lid120min'MDA and MPO values were higher than ischemia group, results had significant difference(P<0.01). but MDA and MPO Lid30min values and ICAM-1 each time point values compared with ischemia group, the results were not significant (P>0.05).(4) Lidocaine pretreatment group each time point results group compared: Lid30min'ALT values compared with Lid60min and Lid120min'values, results were significantly different(P<0.01), Lid60min'values compared with Lid120min had no significant difference (P>0.05); MDA, ICAM-1 and TNF-αvalues, group compared results had no significant difference (P>0.05); but MPO three time point values, group compared, in addition to Lid30min'values compared with Lid60min had significant difference(P<0.01). the other results had no significant difference (P>0.05).Conclusion:In the young SD rats, intestinal ischemia-reperfusion induced acute liver injury, with the extent of ischemia-reperfusion time, the damage gradually increased. After intervention of lidocaine young rats of ischemia-reperfusion liver pathological changes were reduced on different levels. And intervention of lidocaine reduced serum ALT values, liver tissue MDA levels, MPO levels, ICAM-1 and TNF-a activity levels, that showed lidocaine of protective effect against liver damage, but its protective mechanism on the liver to be further study. |
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