Protective Effects Of Lycopene On GTW-induced Acute Hepatic Injury In Mice

Objective:To explore the protective effect of lycopene on GTW-induced acute hepatic injury in mice.Methods:(1) Model building:40 mice were divided into 4 groups in random: control group,model group,lycopene high dose group(20mg·kg^-1) and lycopene high dose group(10mg·kg^-1).The rat models of hepatic injury were established by twentyfold(270mg·kg^-1) after 6d prophylactic administration.(2) Determination:ALT and AST activities in serum,SOD and MDA in liver,and histopathological examination were detected to study the hepatoprotective effect of lycopene.Results:lycopene showed significant protection against the hepatotoxic actions of GTW,markedly alleviated AST,ALT level in serum; enhanced SOD,reduced MDA in liver,liver index, and reduced hydropic degeneration. Compared with GTW-induced acute hepatic injury of mice model group: (1)Lycopene decreased the serum activities of AST,ALT(P<0.05);(2)Lycopene diminished significantly MDA contents(P<0.01)and increasedthe activities of SOD(P<0.01)in serum and liver tissue;(3)Lycopene reduced the liver index(P<0.05);(4).Degenerationand necrosis of liver tissues were abated and pathological changes were relieved.Conclusion:(1) GTW has strong liver toxicity,Lipid peroxidation of free radical and apoptosis induced by tripterygium glycosides is one of the pathological mechanism of GTW-induced acute hepatic injury.(2) This paper replicated the hepatic injury model successfully.confirmed the 20 times common dose,18 hours as the effective dose and effective time to establish hepatic injury model.(3) Lycopene has protective effects on hepatic injury by GTW in mice,the mechanisms possibly contribute to its action of antioxidation.