Font Size: a A A

Effect And Mechanism Of Ursolic Acid On The Proliferation Of Vascular Endothelial Cells

Posted on:2012-01-08Degree:MasterType:Thesis
Country:ChinaCandidate:W LiFull Text:PDF
GTID:2154330335986661Subject:Pathology and pathophysiology
Abstract/Summary:PDF Full Text Request
Purpose: To investigate the effects of the expression of VEGFR2(KDR), ERK1, the phosphorylation of ERK1(p- ERK1), c-Jun and CyclinD1 of Human Umbilical Vein Endothelial Cells(HUVECs) treated with ursolic acid, and to explore the effect and mechanism of ursolic acid on the proliferation of HUVECs.Methods: HUVECs were treated with ursolic acid at 0, 5, 10, 20, 40, 80μmol/L for 36h, or treated with ursolic acid at 20μmol/L for 0, 12, 24, 36, 48h for the course assay in vitro. MTT and typan blue staining were performed to evaluate the effect of ursolic acid on the growth of HUVECs.Western blot analysis was applied to investigate the protein expression of ursolic acid on the phosphorylation of KDR, ERK1, p-ERK1, c-Jun, and cyclinD1. Immunofluorescence analysis was used to examine the protein expression and localizations of ERK1, p-ERK1 and KDR. The protein expression and translocation of ERK1 were studied by confocal laser scanning microscopy (CLSM).Results: Ursolic acid inhibited the growth of HUVECs in dose- and time-dependent manners(P<0.05). The median inhibition concentration (IC50) value for 36 h treatment was 20μmol/L. Western blot results showed that ursolic acid suppressed the expression of KDR, ERK1, p-ERK1, c-Jun , and CyclinD1 in a time-dependent manner(P<0.05). Immunofluorescence analysis results indicated that the fluorescence intensity of the expression of ERK1,p-ERK1 and KDR in HUVECs after treated with ursolic acid was obviously weakened compared with the control groups, and the attenuation trend was in a time-dependent manner. It's showed that the fluorescence intensity of the protein of ERK1 was obviously weakened than the blank control group. As time increased, the fluorescence intensity of ERK1 expressed in nuclei was also weakened.Conclusion: Ursolic acid could inhibit the proliferation of HUVECs by suppressing the ERK pathway.
Keywords/Search Tags:Ursolic acid, HUVECs, ERK signal transduction pthway
PDF Full Text Request
Related items