The Effect Of Octerotide On Proliferation And Apoptosis In Human Colonic Cancer Cell SW480

Objective: To study the effects of octreotide on proliferation,apoptosis and cell cycle in human colonic cancer line SW480 in vitro,and to explore the effects of octreotide on the expression of apoptotic related genes fas and survivin in human colonic cancer cell,and further to explore the possible mechanism of octreotide inducing apoptosis.Methodes:1 Cell culture of human colonic cancer line SW480.2 Human colonic cancer line SW480 were incubated with different concentration of octreotide in vitro.MTT assay were used to determine the influence of octreotide on proliferation of human colonic cell line SW480.3 The influence of octreotide on apoposis of human colonic cell line SW480 was determine by flow cytomery.4 The influence of octreotide on cell cycle of human colonic cell line SW480 was determine by flow cytomery.5 Human colonic cancer line SW480 were incubated with different concentration of octreotide in vitro ,and the expression apoptotic related genes Fas,survivin were measured by flow cytomery.6 The expression apoptotic related genes fas,survivin mRNAwere measured by RT-PCR,and undertaked semiguantitative analysis by computer aided video system.Results:1 The proliferation of human colonic cancer line SW480 could be inhibited by different concentrations of octreotide(0.5ug/ml,1 ug/ml,2 ug/ml,4 ug/ml,8 ug/ml,16ug/ml).In certain concentrations range,with the increasing concentrations of octreotide and the extending time of trestment,the inhibitory effects were enhanced,and there was a significant different between the octreotide group and the control group (P<0.01).The inhibitory effect of octreotide at 8 ug/ml was the most siginificant,the inhibitory effect of octreotide at 16ug/ml was weaker than 8 ug/ml. Octreotide could inhibition the proliferation of SW480 and this inhibitory was dose-dependent and time-dependent.2 Octreotide(0.5ug/ml,2ug/ml,8ug/ml)can induce apoptosis in human colonic cancer cell line SW480, apoptosis rate were 2.120±0.116%,4.350±0.251%,6.520±0.490% in 48 hours. With the increasing concentrations of octreotide,the apoptosis rate were enhanced,and there difference had statistical siginificance compared the control group (P<0.01).3 G1 phase rate gradually increased,S phase rate gradually increased,cell cycle was arrested at G1 phase in human colonic cancer line SW480 treated by octreotide(0.5ug/ml,2ug/ml,8ug/ml) for 48 hours, the difference had statistical siginificance compared with the control group(P<0.05). G2 phase rate was not changed in human colonic cancer line SW480 treated by octreotide, the difference had no statistical siginificance compared with the control group(P>0.05).4 Octreotide(0.5ug/ml,2ug/ml,8ug/ml)can increased the mRNA and protein expression of fas,and reduce the mRNA and protein expression of survivin in human colonic cancer cell line SW480 for 48 hours, the difference had statistical siginificance compared with the control group(P<0.05).Conclusions:1 In this study,MTT assay show that the growth of human colonic cancer cell line SW480 cells could be inhibited by octreotide obviously and the inhibition of octreotide did not steadily increase with the increasing dose.It suggests that effect of octreotide is achieved via somatostatin receptors.2 Octreotide can induce apoptosis and arrest cell cycle at G1 phase in human colonic cancer cell line SW480,it has a dose-dependent and time-dependent relationship.3 Octreotide can increase the mRNA and protein expression of fas,and reduce the mRNA and protein expression of survivin in human colonic cancer cell line SW480.4 Apoptosis induce by Octreotide may be related with arresting cell cycle at G1 phase, increasing the expression of fas and decreasing the expression of survivin in human colonic cancer cell line SW480.