The Infiuence Of Salidroside On Energy Metabolism To Hypoxia-induced Cardiomyocyte

Myocardial cells during hypoxia is always accompanied by changes in energy metabolism,Including the use of metabolic substrate,Production structure. Changes in energy consumption structure.Energy metabolism under hypoxia hypoxic myocardial cell injury may be an important mechanism, energy storage Reduced. Energy consumption Increased,accumulation of metabolites are increased hypoxic injury of myocardial cells. So for anoxic environment can promote cellular energy metabolism of drugs or the means have become the concerns of the medical profession.Salidroside as an effective plant extracts of Rhodiola. The nature of sweet,bitter,flat,with yiqihuoxue. The effectiveness of asthma Tongmai,For the treatment of qi and blood stasis. Chest pain.,Burnout asthma and La poison (high altitude reaction) embolism. Salidroside also has a variety of physiological. Pharmacological activity,with anti-ischemic,Anti-hypoxia, antagonist Ca²⁺,Free Radical Scavenging,Anti-aging effect.In recent years,There is growing recognition salidroside important role in cardiovascular disease. Many studies have confirmed that salidroside under different conditions to improve energy metabolism in mice;Myocardial ischemia - reperfusion, hypoxia, lack of protective effect of myocardial glucose.Salidroside and its preparation have been widely used in clinical. Especiall in cardiovascalar-disease,For example,coronaryheart-disease,hypertension,heart-failure,arrhythmia,cardiomyopathy etc. However, the impact of their specific ways has still not clear.To further investigate the effects on myocardial salidroside. In this study the level of observation from cell salidroside myocardial cells by hypoxia. From the energy point of view of science salidroside oxygen metabolizing enzyme activity and its related protein expression,Chinese medicine for the prevention and treatment of coronary heart ischemia, hypoxia disease reference.Objective:1 Primary culture of SD (Sprague-Dawley) rats,neonatal rat cardiomyocytes. Copy the hypoxic rat model. Salidroside different concentrations in the supernatant of hypoxic cardiomyocytes of LDH;2 Salidroside observed SDH,CPT-1 in hypoxic myocardial cells ;3 Salidroside observed CaM in hypoxic myocardial cells the expression ;4 Study Salidroside improvement of energy metabolism in hypoxic myocar- dial cells may be ways;Methods:1 Newborn SD rats of 1-3d,Isolated cardiac cells cultured in vitro, Injury model to establish myocardial hypoxia , demand in accordance with experiment were divided into normal control group, Hypoxic injury model group. Salidroside low,medium and high doses (10,50,100μg / mL) Intervention group;2 Success of cardiac cell culture,and the establishment of hypoxia model, the myocardium was detected by colorimetric lactate dehydrogenase supernatant LDH and intracellular SDH)of the activity;3 With ELISA kitDetection of intracellular the activity of CPT-â… ;4 With Westren Blot Detection the expression of CaM of myocardial cells;Results:1 1.1 Hypoxia led to increased activity of LDH in the culture medium:LDH in the hypoxic group decreased in the supernatant there was a significant difference Compared with normal group (P <0.05). Hypoxia plus of Medium and high dose drug group LDH activity was reduced after 3h. There was a significant difference Compared with the hypoxic group (P <0.05). Hypoxia group compared with the low-dose Sal. Reduction in LDH was no significant difference. 1.2 hypoxia led to SDH activity decreased intracellular:the decrease of SDH in Hypoxia group Compared with the normal group was significant difference(P<0.05);(medium , large) dose group of oxygen after 3h ,SDH activity was significantly higher than the hypoxic group, there was significant difference (P <0.05);the low dose Sal group compared with hypoxia group, SDH was no significant difference in the increase of (P> 0.05). 1.3 LDH decrease and SDH increase there was concentration with the dose-dependent.2 Hypoxia leading to CPT-â… a ctivity decreased in cell ; Hypoxia group CPT-â… decrease compared with the normal group was significant difference (P <0.05). (Small, medium, large) dose group of oxygen 3h dosing the CPT-â… activity increased .However, compared with hypoxia group there was no significant difference (P> 0.05).3 Hypoxia led to decreased expression of intracellular CaM: Hypoxia group, the expression CaM of intracellular significantly decreased compared with the normal group, there was significant differences (P <0.05).(Small, medium, large) dose group 3h after hypoxia the expression of CaM increased;compared with the hypoxic group the significant difference (P <0.05), Compared different concentrations of SAL expression of CaM increased, dependent dose.Conclusion:1 Salidroside can reduce the activity LDH supernatant of myocardial hypoxia.2 Salidroside can increase the activity SDH of hypoxic myocardial cells3 Salidroside on CPT-1 of activity hypoxic myocardial cells was not signi- ficant.4 Salidroside can increase the expression CaM of hypoxic myocardial cells5 Salidroside improve hypoxia myocardial energy metabolism pathway. There was connection with increased SDH activity, increase the expression GaM.6 Salidroside improve hypoxia myocardial energy metabolism pathway.There was no connection with increase CPT-1 activity.