| ObjectiveChinese herbal formula "zhiganfang" is the experience prescription of Professor Zhou Fusheng, After many years for clinical observation, we can find that Chinese herbal formula "zhiganfang" can significantly improve the clinical symptoms of NAFLD, it can regulate metabolism of TG, improve liver function and no adverse reactions. This topic attempts to discuss the possible mechanism of adiponectin and resistin for Chinese herbal formula "zhiganfang" by establishing non-alcoholic fatty liver rat model.Methods48 SD rats were randomly divided into a control group (one death in collected samples causing by narcotic overdose), of which 12 weeks of high fat diet rat NASH model, after the success of stochastic modeling, separation 8 of model group, the 32 successful model of SD rats were randomly into "zhiganfang" high dose group, middle dose group, low dose group and western medicine control group. ELISA assay for resistin and adiponectin.Results(1) body weight compared with normal group, model group P<0.05, the difference was statis-tically significant; with the model group, western medicine group, "zhiganfang" high, medium and low groups were P>0.05, the difference was not statistically significant. (2) liver weight in rats compared with normal group P<0.05, the difference was statis-tically significant; with the model group, western medicine group, "zhiganfang" high, medium and low groups were P>0.05, the difference was not statistically significant.The correlation analysis showed that it was linear correlation between the liver weight and body weight of rats (3) light microscopy of lipid droplets containing rat hepatic lobule cells/total cell number, compared with normal group, model group P<0.05, the difference was statis-tically significant. Compared with model group, rats in each group were significant differences (P<0.05)except Western medicine group; Compared with the "zhiganfang" low dose group, " zhiganfang " high dose group P<0.05,the differences were Statistical significance; and compared with "zhiganfang" middle dose group, "zhiganfang" high group P<0.05, the differences were Statistical significance. And low dose group P>0.05, the difference was not statistically significant. (4)compared with model group, in addition to Western medicine group, the other rats in each group differences in liver inflammation were significant (P<0.05). (5) plasma Adiponectin:compared with normal group, model group P<0.05, the difference was statis-tically significant compared with model group; compared with the model group, western medicine group, "zhiganfang" high, medium and low dose groups were P<0.05, the differences were Statistical significance; compared with western medicine group, "zhiganfang"low dose group were P<0.05, there were significant difference; "zhiganfang" high dose group, middle dose group, P>0.05, the difference was not statistically significant. (6) Plasma Resistin:compared with normal group, model group P<0.05, the difference was statis-tically significant compared with model group;Compared with the model group, western medicine group, "zhiganfang" high, medium and low dose groups were P<0.05, there were significant difference;Compared western medicine group, the "zhiganfang" low and medium dose group was P<0.05, there was significant difference; "zhiganfang" high-dose group, P>0.05, the difference was not statistically significant.; Compared with "zhiganfang" low dose group, medium and high dose group P>0.05, the difference was not statistically significant.Conclusions(1)Adiponectin and resistin are closely related to non-alcoholic fatty liver disease. (2) "zhiganfang" can improve lipid droplets in rat hepatic lobule containing cells/total cells, and it also can increase Adiponectin expression and decrease the expression of Resistin. (3) Chinese herbal formula "zhiganfang " is an effective prescription for the treatment of non-alcoholic fatty liver. Part of the mechanism may be related to adiponectin and resistin. |
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