The Effect Of Formulae That Can Tonify Qi And Dissolve Stasis On IL-1, TNF-α, 6-K-PGF_1α, TXB₂ Of Rats Model Of Postpartum Endometritis

Objective To explore the effect of formulae that can tonify Qi and dissolve stasis ontumor necrosis factor-alpha (TNF-α), interleukin-1(IL-1), 6-keto-prostaglandin F1alpha(6-K-PGF1α), thromboxane B2(TXB2) of rats model of postpartum endometritis and the action mechanism of promoting postpartum involution of uterus.Methods Seventy-two SD pregnant rats were randomly divided into 6 groups:blank group,model group, the comparing group with Chanfu Kang grain used and small, middle, large dose of formulae tonifying Qi and dissolving stasis group. The model of subinvolution of uterus was established on rats in groups beside the blank one. All rats in each group were administered by intragastric infusion with corresponding drugs during day 3～10 after the model established. The content of IL-1, TNF-α, 6-K-PGF1αand TXB2 were respectively detected by the method of Immunohistochemistry(IHC), Enzyme-Linked Immunosorbnent Assay (ELISA) and Radioimmunoassay(RIA).Result1.The formulae that can tonify Qi and dissolve stasis, based on the "Tonifying Qi and Dissolving Stasis Method"can change the pathological image of the uterus of the rats with postpartum endometritis caused by postpartum inflammation, and alleviate the inflammatory reaction. 2.The formulae that can tonify Qi and dissolve stasis can alleviate the TNF-a value in rats with postpartum endometritis.TNF-a value in rats in modeling group has obvious growth, compared with the blank group (P<0.01). As to the comparing group with Fuchan Kang grain used, the high dose group with formulae tonifying Qi and dissolving stasis, compared with the modeling group, has a decreasing value of TNF-a value, which is of significance (P<0.05). There's no significance in the high dose group with formulae tonifying Qi and dissolving stasis used and comparing group with Chanfu Kang grain used(P>0.05). Also there's no significance between and among all figures in the high dose group with formulae tonifying Qi and dissolving stasis used(P>0.05).3. The formulae with functions of tonifying Qi and dissolving stasis can weaken the IL-1 value in rats with the postpartum endometritis. Rats in modeling group embark significant differences in the average grey level and areal density level, compared with the blank group (P<0.01). As to the comparing group where Chanfu Kang grain is in use, in the formulae that tonifying Qi and dissolving stasis, high volume group, comparing with the modeling group, the average grey level increased, and the areal density level decreased, both with statistical significance (P<0.05); the comparing group with Chanfu Kang grain used and the high dose group with formulae tonifying Qi and dissolving stasis, shared the decline in areal density level (P<0.01). There's no significance in the high dose group with formulae tonifying Qi and dissolving stasis used and comparing group with Chanfu Kang grain used(P>0.05). Also there's no significance between and among all figures in the high dose group with formulae tonifying Qi and dissolving stasis used(P>0.05).4. The formulae that can tonify Qi and dissolve stasis can decrease the value of 6-K-PGF1a and increase the value of TXB2.The modeling group, compared with the blank group,has significant difference in 6-K-PGF1a value and TXB2 value (P<0.05,P<0.01).Comparing group with Chanfu Kang grain used has a significant increasing value in TXB2 when compared with the modeling group (P<0.01).The high dose group with formulae tonifying Qi and dissolving stasis used, compared with the modeling group, has a decreasing value of 6-K-PGF1a and increasing value of TXB2, which is of great significance (P<0.05), and TXB2 value has tremendous increase (P<0.01).There's no significance in the high dose group with formulae tonifying Qi and dissolving stasis used and comparing group with Chanfu Kang grain used in TXB2 value (P>0.05).Conclusion The formulae that can tonify Qi and dissolve stasis can change the pathological image of the uterus of the rats with postpartum endometritis caused by after birth inflammation;also it can weaken the value of inflammatory factor IL-1,TNF-a, with significant anti-inflammatory action;it also can decrease the value of 6-K-PGF1a, increase the TXB2 value and adjust the proportional relation PGI2/TXA2, promoting the dynamic balance of PGI2/TXA2, enabling smooth blood circulation and vanishing the stasis. Through all these adjustive functions, the formulae that can tonify Qi and dissolve stasis can effectively cure postpartum endometritis, thus stimulating involution of uterus after delivery. The effect of middle, high dose group with formulae tonifying Qi and dissolving stasis used were apparent, especially the high one.