The Expression And Significance Of CD133 And Survivin In Different Pathological Status Of Gastric Mucosa

Objective To analysis the expression of CD 133 and Survivin in chronic gastritis, intestinal metaplasia, atypical dysplasia and gastric cancer, the effect of Helicobacter pylori and clinical factors. To explore the function, relationship and clinical significance of CD 133 and Survivin.Methods 115 patients were enrolled in the study from endoscopy room of the General Hospital of Tianjin Medical University Hospital from February 2010 to October 2010. They were divided into groups of chronic gastritis, intestinal metaplasia, atypical dysplasia and gastric cancer according to pathology. Patients were first diagnosed without preoperative chemotherapy or immunotherapy in gastric cancer group, who were treated by surgery after diagnosis and follow-up of their postoperative conditions. Helicobacter pylori was detected by13 C Urea Breath Test in all patients. Acording to the result, they were didvided into positive and negative group. CD 133 and Survivin were detected by immunohistochemical method and further were anlysised these changes and relation with age, sex, degree of differention, TNM stage, lymph node metastasis in gastric cancer and their relevance.Results 1. The composition of sex and age was no significant difference in different groups.2. The expression rate of CD133+ was 6.7%,30.0%,36.7%,77.5% respectively in chronic gastritis, intestinal metaplasia, dysplasia and gastric carcinoma group; the positive rate of Survivin was 0%,6.7%,33.3%,62.5% respectively. It was shown a tendency of progressive increase.3. The expression level of CD 133 and Survivin were significantly higher in Helicobacter pylori (Hp) positive group than negative group (P＜0.05).4. The expression of CD 133 and Survivin were not correlated with age and gender in gastric cancer patients (P＞0.05). The expression of CD133 was correlated with tumor differentiation, lymph node metastasis (P<0.05) but not with TNM stage (P＞0.05); The expression of Survivin was correlated with differentiation and TNM stage of gastric cancer (P＞0.05), but not with lymph node metastasis (.P>0.05).5. There was a positive correlation between CD 133 and Survivin in gastric cancer (r=0.687).Conclusions 1. Survivin and CD 133 upregulated in the development of gastric cancer suggested that Survivin and CD133 played an important role in the development of gastric cancer possibly.2. The level of CD133 expression gradually increased in the stage of intestinal metaplasia and atypical dysplasia appeared; The expression of Survivin in atypical dysplasia appeared to increase, which suggested that early intervention for gastric cancer molecular markers.3. Helicobacter pylori (Hp) may be one of the factors which induced upregulation of CD133 and Survivin.4. The expression of CD133 was significantly correlated with differentiation of cancer and lymph node metastasis, but not with age, gender and the TNM stage. The expression of Survivin was significantly associated with differentiation of cancer and TNM stage. 5. The expression of CD133 and Survivin were positively correlated, which suggested they could promote the expression each other through direct or indirect way and had a synergistic effect.6. CD133 and Survivin may become the gene targets for treatment and early intervention of gastric cancer and precancerous lesions through genetic engineering technology.