Experimental Study On The Compatibility Between Chitosan-gelatin Scaffold And Synovium-derived Mesenchymal Stem Cells From Human

Objective to investigate the compatibility between chitosan-gelatin scaffold and synovium-derived mesenchymal stem cells(SDSCs).Material and methods Mixed synovial cell population(MSCP) were released from synovial membranes by enzymatic digestion and synovial derived mesenchymal stem cells(SDSCs) were isolated from MSCP by passage isolation. Chitosan and chitosan-gelatin(chitosan/gelatin=1:1) scaffolds were fabricated by freezing and lyophilization, and we evaluated their degradation kinetics, weight loss, water uptake, porosity and density, and then SDSCs were seeded on chitosan-gelatin scaffold and its chitosan control. Cytocompatibility was detected with scanning electron microscopy(SEM) and MTT assay at day 3,7 and 10 after seeding.Results Passage isolation shows large, flat, spindle-shaped or stellate-shaped cells with positive markers of CD44, CD90 and CD 105 that their positive rates were 98%,94% and 49% respectivly. Scaffolds(chitosan/gelatin=1) were characterized by a highly porous structure with interconnected pores that their size was in the range of 50μm～180μm, and their porous structure was different from pure chitosan from the viewpoint that the surface area of pore membrane around each pore appeared to be wider and thinner compared with pure chitosan scaffods. SEM image of scaffold seeded with SDSCs showed that SDSCs spreaded well and homogenously distributed throughout the entire chitosan-gelotin scaffold, MTT assay observed that the A value of cells in chitosan-gelotin scaffold was followed as day 10 (1.43±0.11)>day 7 (1.35±0.08)>day 3 (0.43±0.08)(P<0.05), and it was higher than that of its pure chitosan control (day3:0.1352±0.0715, day7:0.2710±0.0741, day10:0.4936±0.0671) (p<0.05)Conclusion The chitosan-gelatin scaffold is much more compatible with SDSCs than its control.