Experiment Research Of Bushentianjingfang Effect On Hemopoietic Progenitor Cell CFU-GM,CFU-E In Mice With Aplastic Anemia

Objective:To investigate the effects of Bushentianjingfang on Bone marrow hemopoietic progenitor cells and Bone marrow pathology and Peripheral blood cells in mice with Aplastic Anemia. Methods:Mading mouse model of aplastic anemia by injecting kunming mice on Abdominal with Cyclophosphamide and on Subcutaneous with benzene-cornoil,affert mading successful medle mice,giving the above mice bushentianjingfang suspension for 35days, the blood was taken from theorbital arteriovenous and peripheral blood cells were counted; The mices were sacrificed under the sterile condition, left femur was taken and Bone marrow mononuclear cells was prepared, the number of bone marrow mononuclear cells(BMNC) were counted. CFU-E and CFU-GM were measured by methylcellulose seni-solid colony culture,to observed the effect of bushentianjingfang on hematopoietic progenitor cells in mice. Results:RBC Hb WBC PLT in bushengtianjingfang high and middle dosage treated group were higher than that in the AA group (P<0.05); BMNC CFU-E CFU-GM in bushengtianjingfang treated group were higher than that in the AA group, there is differences between bushengtianjingfang high and low dosage treated groups (P<0.05),but on in high and middle group(P> 0.05);Model group mice marrow cavity was filled with fat tissue and hematopoietic structure was damaged. The number of fat cells were decreased and three series cells were increased,the high dosage group was significant.Conclusion:Bushentia-njigfang can improved the numbers of hemopoietic progenitor cells and peripheral blood cells.at the same time, hematopoietic tissue was repaired;Bushentianjingfang played a role with the influence of hemopoietic progenitor cells.