The Research Of The Killing Effective Of Carbon Nanoparticles Conbined With THP On Bladder Cancer

Objective: To construct the ACNP-THP conjugation and to investegated the Cytotoxicity ACNP on bladder cancer cell lines BIU-87.Methods: we actived carbon nanoparticles and Pirarubicin under the condition of sonation and add amine groups to NP and THP. We conjugated the actived NP and THP under the same condition.Results: We constructed ACNP-THP conjugation successfully. And ACNP showed toxic free to bladder cancer cell line BIU-87.Conclusion: We constructed ACNP-THP conjugation successfully. And ACNP showed toxic free to bladder cancer cell line BIU-87.PartⅡTo construct bladder cance model with NMU, and to explore the possible mechanismObjective: to construct bladder cancer in situ model with NMU and to investigated the possible mechanism of NMU induced bladder cancer. Methods: 60 SD Rats were devided into experimental group and control group at random.NMU was used to intravesical perfuse per 2 weeks at the dose of 2mg for 8w in experimental group,while saline was used in control group. All rats was sacrificed to get the bladder at the end of 8w. All bladders were stored in negative 80°liquid nitrogen. Ras was detected by immunohistochemistry,and PLCεmRNA was detected by RT-PCR.Results: Tumor appeared in 36 rats out of 50 rats in experimental group . Totally tumor formation rate was 81.82%. While there is no tumor appeared in control group.Ras protein was positive in 32 rats in experimental group by immunohistochemistry, while negative in control group. PCR showed PLCεmRNA was positive in experimental group,while negative in control group.Conclusion: MNU could induce the oncogenesis in bladder. The possible mechanism was MNU make Ras actived and PLCεoverexpressed.PartⅢTo investigated the cytotoxicity of ACNP-THP in vitro and in vivoObjective: to investigate the anti-cancer efficacy of ACNP-THP conjugation on bladder cancer in vitro and vivo.Methods: bladder cancer cell line BIU-87 were plated in culture. ACNP-THP, THP , ACNP and saline were added in the culture. FCMwere used to evuluated the anti-cancer effection. ACNP-THP, THP and ACNP were injected into the constructed bladder cancer model once a week ,totally for four weeks. The bladders were harvested at one hour after the first installation and after four installations. The anti-cancer efficacy were observed.Results: ACNP-THP showed the highest anti-cancer effects(p<0.01), THP showed anti-cancer effects(p<0.01). And ACNP only showed the same anti-cancer effects with saline (p>0.05). ACNP-THP installation group could increase more cancer cell death rates than THP only group by FCM(p<0.01); THP could increase more cancer cell death rates than ACNP group by FCM ( p<0.01 ) ; ACNP only hardly induce cancer cell death(p>0.05).One month after the installation, ACNP-THP conjugation could decrease the tumor volume , meanwhile, increase the tumor necrosis. The THP only appeared no tumor depression at all.Conclusion: ACNP-THP conjugation could increase the killing efficacy of THP, while THP only seems toxic free to the tumor.