Effect Of Interference Of MTA1 By Shrna On T He Proliferation,Apoptosis And Expression Of Related Proteins In Breast Cancer Cell

Objective:To silence the expression of Metastasis-associated gene 1 (MTA1) in breast cancer cell line MDA-MB-231 by using short hairpin small interfering RNA (shRNA) and observe its effects on expression of 15- lipoxygenase -2(15-LOX-2), p53 and bcl-2 proteins.Methods:The shRNA-MTA1 plasmid was stably transfected into MDA-MB-231 cells and the cell proliferation was evaluated using MTT assay. The cell cycle distribution and apoptosis were analyzed using flow cytometry. The mRNA and protein expression levels of MTAl, 15-LOX-2, p53 and bcl-2 were determined using RT-PCR and Western blotting,respectively.Results:shRNA-MTAl significantly suppressed the expression of MTA1 gene in MDA-MB-231 cells, inhibited the proliferation,induced apoptosis,and arrested the cell cycal in G1 phase. The difference was significant compared with control group(P <0.01). Expression levels of 15-LOX-2 and p53 were significantly up-regulated, but MTAl and bcl-2 were significantly down-regulated in MDA-MB-231 cells in shRNA-MTAl group compared with the blank control group and Negative control group (P <0.01).Conclusions:Silencing MTA1 gene inhibited the proliferation and induced the apoptosis of MDA-MB-231 cells. This effect may be related with up-regulation of the expression of 15-LOX-2 and P53 and down-regulation of bcl-2.