New Nano–composite Materials To Dog Alveolar Bone Defects Experimental Study

Objective Evaluation of new nano-composite materials in experimental animals dog periodontal tissue regeneration.Methods Selection of 6 adult mongrel dogs, in the mandibular premolar and first molar buccal surgical preparation 3 to 5mm×5mm×5mm of alveolar bone defects. Latin square design with the establishment of two experimental groups and one control group, A group: new nano-composite materials and implantation of autologous platelet-rich plasma (PRP) mixture, PRP contains several growth factors, can be effective in promoting periodontal tissue regeneration, but it also has a certain adhesion, PRP is activated into a gel, and the materials mixed together to form a soft tissue scaffold similar to the biological material, the material can prevent the early shift, simple, effective reduction the material loss. B group: implantation of new nano-composite materials; C group: do not put any material, direct suture; respectively after 2 weeks, 4 weeks, 12 weeks by gross observation, X-ray plain film and Histological observation,Evaluation of new nano-composite materials on dogs effect of alveolar bone defects.Results Gross observation showed that A,B,C group after 2 weeks no significant change in bone defects, A,B groups there is also some composite materials; after 4 weeks and 12 weeks, gradually narrowing the scope of bone defects, new bone tissue can be seen ; C group 4 weeks after the defect did not change significantly after 12 weeks, the defect can be seen a small amount of new bone tissue, little effect. X-ray findings with the time A,B groups, bone defects of the shadow faded, showing significant trabecular bone structure, A group than the repair effect of B group , and C group the defect of the shadows gradually reduced, but the trabecular bone structure is not obvious, and the surrounding normal tissue defects in the edge of the boundary was; Histological observation of A,B groups after 4 weeks, a large number of defects of alveolar bone and significant new trabecular bone structure, bone trabeculae in close order, Over time, 12 weeks, trabecular bone density changes in the thick, A group of mostly trabecular bone has been integrated into the film, B group showed a trabecular network connected to each other most of the newborn with the surrounding trabecular bone; There are many osteoblasts, A Group was significantly better repair than B Group; C group after 4 weeks, proliferation of fibrous connective tissue, a large number of inflammatory cell infiltration, local as well as "sequestrum "appears, the effect is not obvious.Conclusion The novel nano-composite materials can be effective in promoting periodontal tissue regeneration, materials, adding autologous platelet-rich plasma, the effect is more evident.