| Objective The model of diabetic nude mouse was established in this study. BrdU-labelled human dermal-derived multipotent stem cells were engrafted into the wound of the nude mice by means of injection. After designing control group, the differences of two groups of wound healing nude mice were compared by statistical analysis. The distribution and differentiation of human dermal-derived multipotent stem cells were observed in order to evaluate the influence on wound healing in diabetic nudemouse.Methods (1)Establishment of the nude mouse model: the use of streptozocin(STZ) by intraperitoneal injection once; (2)Separation, culture and purification of human dermal-derived multipotent stem cells; (3)Calculating of the rate of wound healing: application of a transparent membrane and statistical analysis to the differences; (4)Histopathological observation of the wound tissue was completed by Hematoxylin-eosin (HE) staining; (5)The distribution and differentiation of human dermal-derived multipotent stem cells: the specimens around wound were immunohistochemically stained and cell differentiation was observed in serial section.Results (1)The successful construction of the wound model of diabetic nude mouse; (2) human dermal-derived multipotent stem cells were isolated, purified and cultured successfully; (3)According to statistical significance, human dermal-derived multipotent stem cells increased significantly the rate of wound healing in nude mouse; (4)Compared with control group, the newborn epidermis of experimental group was thicker, cell layers and the layer were more obvious. Basal cells were arranged of single-layered columnar structure, combined tightly and integrated into organizational structure. (5)BrdU positive cells with brown nuclei aggregated in the epidermis, dermis and hypodermia. Some positive cells with brown cytoplasm appeared in the expressed keratin simultaneously in serial sections.Conclusion BrdU-labelled human dermal-derived multipotent stem cells were injected into the wound of the nude mice. These cells have the potential for differentiating into epidermal cells under the microenvironment of diabetic wound. Human dermal-derived multipotent stem cells can improve wound healing rate and promote diabetic wound healing. |
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