Correlative Study On TLR4 , Hs-CRP In Peripheral Blood And Subclinical Chorionic Amniotic Membrane Inflammation Of Premature Labor

Objective:Discuss the relationship of Toll-like receptor 4 and hs-crp level with Peripheral Blood of subclinical chorionic amniotic membrane inflammation of Premature Labor and analyze early diagnosis of value of TLR4 and hs-crp expression in subclinical chorionic amniotic membrane inflammation of Premature Labor .Methods: Placenta and fetal membranes were pathologically analyzed in 47 cases of premature labor (28--36⁺⁶weeks),which was divided into subclinically infected group (22 cases), namely subclinical chorionic amniotic membrane inflammation person; and non-infected group (25cases) namely without chorionic amniotic membrane inflammation person. excluding clinical chorionic amniotic membrane inflammation person. taking normal pregnant woman (20 cases) as control.(1)The expression rate of TLR4 in CDl4 and TLR4 average fluorescence intensity on monocytes was determined by flow cytometry;(2)Hs-crp level in serum was measured by latex enhancement immunity than turbidity method.(3)The change of blood was measured by blood routine test.Results : (1)The expression rate of TLR4 in CDl4 was 56.299±8.234% ,36.505±5.195% , 22.089±5.846% respectively in subclinically infected group ,non-infected group and normal contol. TLR4 average fluorescence intensity on monocytes was 53.045±7.780,33.633±4.768,17.290±4.449 respectively. Both of subclinically infected group are significantly higher than both of non-infected group and normal control (p<0.05);(2)Hs-crp level in serum was 10.946±4.968 mg/l,4.656±1.291 mg/l,2.121±0.936 mg/l respectively in subclinically infected group,non-infected group and normal contol. There were significant difference in levels of hs-crp between subclinically infected group and non-infected group, Hs-crp level was higher in non-infected group than that in normal control(P< 0.05); (3)The total number leucocyte was 12.695±1.257%,12.600±1.183%,12.735±1.269% of three groups.They were no statistically significant differences(P>0.05); (4)TLR4 average fluorescence intensity correlate positively with the level of serum hs-crp in subclinically infected group(r=0.695,P<0.05).Conclusions:(1)The expressions of TLR4 were markedly increased in subclinically infected group and non-infected group, TLR4 may be play an important role on premature pathogenesis;(2)The levels of hs-crp were markedly increased in subclinically infected group and non-infected group,Hs-crp may be play a role on premature onset or progress; (3) TLR4 average fluorescence intensity correlate positively with the level of serum hs-crp,both can be used as predict early intrauterine infection of premature indicators.