The Experimental Study On The Effect Of Extractive Of Radix Aucklandiae ,Aristolochia Debilis,radix Paeoniae Rubra, Fructus Oryzae Germinates To Streptococcus Mutans In Vitro

Dental caries is one kind of infectious diseases. It was found that S.mutans was the main cariogenic bactera of caries. The study was based on the exsisting experiment of selecting effectively anti-carious ones from 100 natural medicines. The extractives of Radix Aucklandiae, Aristolochia debilis, radix paeoniae rubra and fructus oryzae germinates were chosen as four well-effective medicines to investigate their effects on anti-carious performance, adherence, acid generation, and glucan production of S.mutans .Then the effective ingredients in Radix Aucklandiae and their inhibition of S.mutans were analyzd.The results of this study could provide scientific evidence for further development and enhance the application of natural medicines on caries prevention.Objective:The purpose of this research is to choose well-effective natural medicines from the results of the exsisting experiment, then the medicine experiment in vitro was performed to observe their effects on the growing of S.mutans's for the further study of the effects of natural medicine on caries prevention.Methods: The extractives of Radix Aucklandiae, Aristolochia debilis, radix paeoniae rubra, fructus oryzae germinates from the same batch but different time or different batches were abstracted and tested repeatedly. Coubling dilution method was applied to test the effects of extractives with different concentrations on inhibition of S. mutans and to ascertain the minimum inhibitory concentration (MIC) as well as testing the effect of all the extractives on acid generation, adherence and glucan production of S. mutans. High Performance Liquid Chromatography (HPLC) was applied to analyze the medical ingredients and their contents in Radix Aucklandiae. Then the standard ingredients were tested to observe their effects on the inhibition of S.mutans.Results: 1. Extractives of different concentrations were used to test the inhibition of the growing of S.mutans. It was found that when the extractives of Radix Aucklandiae, Aristolochia debilis, radix paeoniae rubra were no less than 12.5 mg/ml and the extractive of fructus oryzae germinates no less than 6.25 mg/ml, The growing of S.mutans cound be inhibited(P<0.05).2. Extractives of different concentrations were used to test the inhibition of adherence of S.mutans. Results showed that when the separate concentration of the extractives of Radix Aucklandiae, Aristolochia debilis, radix paeoniae rubra, fructus oryzae germinates were no less than 6.25mg/ml, 3.13mg/ml, 3.13 mg/ml and 0.78mg/ml, the adherence of S.mutans were inhibited which had statistical significant difference(P<0.05)with the negative contrast.3. Extractives of different concentrations were used to test the inhibition of acid generation of S.mutans. It was found that when the concentration of the extractives of Radix Aucklandiae and radix paeoniae were no less than 3.13mg/ml, the extractive of Aristolochia debilis rubra no less than 1.56mg/ml and the extractive of fructus oryzae germinates no less than 0.39mg/ml ,the acid generation of S.mutans were inhibited which had statistical significant difference(P<0.05)with the negative contrast.4. Extractives of different concentrations were used to test the inhibition of glucan and insoluble glucan production of S.mutans. Results showed that the extractives of Radix Aucklandiae and radix paeoniae rubra could inhibit the glucan production. When the separate concentration of the extractives of Radix Aucklandiae and radix paeoniae rubra were no less than 6.25mg/ml and 3.13mg/ml, the glucan production of S.mutans were inhibited which had statistical significant difference(P<0.05)with the negative contrast. It was found that the extractives of Aristolochia debilis, radix paeoniae rubra and fructus oryzae germinates can inhibit the insoluble glucan production When the separate concentrations of the extractives of Aristolochia debilis, radix paeoniae rubra and fructus oryzae germinates were not less than 0.78mg/ml, 6.25mg/ml and 0.39mg/ml, the insoluble glucan production of S.mutans were inhibited which had statistical significant difference(P<0.05)with the negative contrast.5. HPLC was applied to analyze the extractive of Radix Aucklandiae. It was found that dehydrocostuslactone (70.6043μg/ml) and Costunolide (36.3432μg/ml) were contained in the extractive of Radix Aucklandiae (2mg/ml) by regression equation. Standard dehydrocostuslactone and Costunolide with different concentrations were used to test the inhitbitive rate of the growing of S.mutans. Results showed that when the MIC of dehydrocostuslactone was 0.441 mg/ml and the MIC of Costunolide was 0.227mg/ml, the growing of S.mutans could be inhibited.Conclusions: 1. The growing of S.mutans could be inhibited by the extractive of Radix Aucklandiae,Aristolochia debilis,radix paeoniae rubra,fructus oryzae germinates .These extractives had different inhibitory effects on adherence,acid generation and glucan production of S mutans.2. Dehydrocostuslactone and Costunolide were found in the extractive of Radix Aucklandiae, which both had inhibitory effect on the growing of S.mutans.