The Level And Clinical Significance Of Plasma And Urine Endothelin-1 In With Henoch-Schonlein Purpura Nephritis

Objective:To observe the change of plasma and urine endothelin-1 (ET-1) levels in children with Henoch-Schonlein purpura nephritis (HSPN) and explore its clinical significance with renal damage, which provide evidences for treatments of HSPN in children.Methods:65 children with HSP were divided into two groups according to the degree of renal damage:35 cases in HSPN group with renal damage and 30 patients in HSP group without renal damage, While 25 cases normal children were used as control group. The levels of plasma and urine ET-1 were detected by double antibody sandwich enzyme-linked immunosorbent assay (ABC-ELISA) before treatment in each group. The levels of 24-hours urine protein and microalbuminuria (malb) were detected by biuret colorimetry and radioimmunoassay turbidimetry, respevtively,and the correlation for urine ET-1 and urine protein. Finally, all data were statistically analyzed by SPSS 17.0.Results:Plasma and urine ET-1 levels were significantly higher in both HSPN and HSP without renal damage patients groups than the control group, (P<0.01); while there was no statistical difference in plasma ET-1 levels between HSPN group and HSP group without renal damage (P> 0.05). Urinary ET-1 levels in HSPN group, however, were significantly higher than those in HSP group without renal damage and the normal control group (P<0.01). 2. The levels of urinary ET-1 were significantly higher in group III(urinary protein>1.0g/24h) than those in groupⅡ(urinary protein 0.15～1.0g/24h) and group 1 (urinary protein <0.15g/24h), (P<0.05 and P<0.01, respectively); Furthermore, urinary ET-1 values were significant higher in groupⅡthan those in groupⅠ(P<0.01).3. The correlation analysis showed that there was a positive correlation between urinary ET-1 levels and urinary malb, urinary ET-1 and the levels of 24-hour urine protein in children with HSPN (r=0.692, r=0.68,P<0.01).Conclusion:Our study demonstrated that ET-1 may be involved in the occurrence and development of the HSPN, Moreover, urinary ET-1 levels could be used as a promising clinical biomarker of renal damage in children with HSPN.