Ge Xia Zhu Yu Tang Yiguanjian Dual Compound Treatment Of Hepatic Fibrosis In Clinical And Experimental Study

Hepatic fibrosis results from the disequilibrium between synthesis and degradation of extracellular matrix (ECM) components. Intense research has established that hepatic stellate cells (HSC) play a central role in the process. During fibrogenesis, quiescent HSC are transformed into myofibroblast-like cells contribute significantly to the process of fibrous scar formation. Recent efforts to understand liver fibrosis focus on oxidative stress.Oxidative stress has been postulated as a important mechanism involved in activation of HSC, which the reactive oxygen species play an important role in the pathogenesis of hepatic fibrosis. In China, hepatic fibrosis, cirrhosis and viral hepatitis most relevant, often because of changes made persistent chronic viral hepatitis, chronic hepatitis and Hepatic fibrosis in pathological features, this paper, based on previous work by CCL4/olive oil-induced Hepatic fibrosis, from pathological observations, serum biochemical markers to further clarify the Ge Xia Zhu Yu Tang Yiguanjian double the effectiveness of liver fibrosis compound; and dual-compound mechanism of liver fibrosis research For the two-compound application for further promotion and development and provide experimental basis.By CCL4/olive oil-induced mouse model of Hepatic fibrosis was observed in mice double compound morphology of liver tissue and liver tissue HA, LN, C-â…£, Pâ…¢NP and HyP. Results:1) Dual compound in the high dose group and middle dose group was significantly improved, fibrous connective tissue in portal area was significantly reduced; the model mice liver lobules, interlobular inflammatory cell infiltration; portal area expanded by fibrous connective tissue hyperplasia was. Dual compound can significantly improve liver fibrosis, the treatment of dual-compound the degree of liver fibrosis model group significantly reduced their care (P<0.01).(2) dual compound 30mg/kg,15mg/kg intraperitoneal injection significantly reduced serum ALT, AST, ALP level, to improve A/G ratio (P<0.01).(3) Dual Compound 30gm/kg,15mg/kg intraperitoneal injection significantly reduced serum liver fibrosis levels of HA, LN, Pâ…¢NP, C-â…£(P<0.01). (4) Compared with model group, hepatic fibrosis in mice during and after the oral administration Hepatic fibrosis in double compound 30mg/kg,15mg/kg,7.5mg/kg can be reduced the expression of TGF-B1, TIMP-1 (P<0.01).2. by CCL4/olive oil-induced mouse model of liver fibrosis, liver tissue preparation were used to detect superoxide dismutase (SOD), malondialdehyde (MDA), hydroxyproline (Hyp) Content.CCL4/olive oil-induced mouse model of liver fibrosis lipid peroxidation in liver tissue MDA content increased, SOD decreased (compared with normal group P <0.01), dual-compound in the dose and the dose were able to reduce the MDA content Increased SOD content (compared with model group P<0.05, P<0.01);. Hyp mouse liver model group was significantly higher (compared with normal group P<0.01), double-dose group and the compound dose group can significantly reduce the Hyp content (compared with model group P<0.01, P<0.05).3. This clinical trial:the 65 Hepatic fibrosis patients were randomly divided into 2 groups to test three months of double-compound and even served as a course of treatment, after treatment, were observed following treatment before and after the change. The results show that:the treatment group after treatment, ALT and AST is almost close to normal levels, TBIL significantly lower than the control group, results suggest that dual-compound on chronic hepatitis B liver function recovery of reduced glutathione than good. The results indicated that the treatment of hepatic fibrosis group than in the control group (PCâ…¢, HA, LN) decreased significantly (before treatment P<0.05).In the text, CCL4/olive oil-induced liver fibrosis model, evaluated the effect of the two-compound treatment of experimental liver fibrosis from serum indicators of liver fiber, content of liver HyP and morphological changes of liver, confirmed that two-compound on CCL4/Olive oil-induced liver fibrosis in mice with a significant therapeutic effect; its mechanism and antagonistic cytokine TGF-1,p inhibited the increase of TIMP-1,and thereby promoting the degradation of collagen. Clinical trials that:Dual compound has delayed liver fibrosis (cirrhosis), the role is the effective drugs to prevention and treatment of chronic hepatitis B patients with cirrhosis.