| BackgroundStudy found that many herbal extracts with anti-tumor effects, anti-cancer mechanism is complicated, such as the induction of apoptosis, including mitochondrial pathway, blocking tumor cell grow cycle, affecting oncogenes and tumor suppressor genes and so on. Research has shown that curcumin can selectively inhibit the growth of various tumor cells and induce apoptosis, the anti-tumor effects and tumor suppressor mechanism has become one of hot research ares.ObjectiveThe main effect of curcumin (Curcumin) on androgen independent prostate cancer cell line DU145 cell growth activity in vitro, cell cycle and induce apoptosis.MethodsEffect of curcumin (10,20,40,80μmol/ml) on androgen-independent prostate cancer cell line DU145 cells in vitro intervention,24h,48h,72h after the four nitrogen a tetrazolium (MTT) colorimetric cell proliferation observed effects, the flow cytometry Determination of cell cycle and apoptosis, the in situ nick end labeling (TUNEL) to detect apoptosis rate, only the cells did not drug as the control group, while observing the other anticancer drugs, such as ginsenosides, Baili Resveratrol, emodin, matrine, tea polyphenols, bicalutamide effects on DU145 cells。ResultsCurcumin (10,20,40,80μmol/ml) in DU145 cells 24h,48h,72h, the cell growth was inhibited and induces apoptosis simultaneously, and showed time and dose-dependent inhibition, apoptosis of each concentration group were significantly higher than the control was significant.Cell cycle G2/M cycle arrest and apoptosis of each concentration group were significantly higher than the control was significant. Other anticancer drugs, such as ginsenosides, resveratrol, emodin, matrine, tea polyphenols, bicalutamide have a similar effect, and induces apoptosis.ConclusionEffect of curcumin on androgen-independent prostate cancer cell line DU145 cell growth inhibition may be through the containment cell cycle and apoptosis to its anti-tumor effect. |
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