| In this study, Phellinus rimosus and Lycopodium japonium have been chemically investigated. Tweenty-eight compounds (1-28), including four new (1-4) steroids, have been isolated by using column chromatography (CC) over silica gel, Sephadex LH-20, RP-C18, MCI gel, SPE and semi-preparative HPLC. Their structures, especially the stereochemistry of the new compounds, were extensively characterized on the basis of spectroscopic methods (IR, UV,1D-/2D-NMR, LR-/HR-ESIMS).Thirteen secondary metabolites, including eleven ergostane-type steroids (1-11) and two cerebrosides (12,13), were isolated from the fruiting bodies of P. rimosus. The new compounds were elucidated to be (22E,24R)-ergosta-7,9(11),22-trien-3β,5α,6β,14α-tetraol (1), (22E,24R)-ergosta-14,22-dien-3β,5α,6α,7α-tetraol (2), (22E,24R)-6-O-ethyl-ergosta-7,22-dien-3β,5α,6β-triol (3), (22E,24R)-15a-O-β-D-glucosyl-3β,25-dihydroxy-5a-ergosta-7,22-dien-6-one (4). Meanwhile,3β-Hydroxy-5a-ergosta-7,22-diene-6-one (5) was a new natural product. The rest were isolated from the genus Phellinus for the first time.Fifteen naturally occurring compounds, including ten triterpenoids (14-23), two steroids (24,25), and three alkaloids (26-28), have been so far identified from the whole plant of L. japonium. Compounds 14-25,27 and 28 were determined as known compounds by comparison of their spectroscopic data with those reported in literature.Evaluation of the cytotoxicities of isolated compounds against a small panel of human tumor cell lines (e.g. MCF-7, HepG 2) is still in progress. |
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