Experimental Study Of Lce Compression Effects On Rats With Spinal Cord Injury

Purpose:Study the effects on edema, hemorrhage, and apoptosis of spinal cord injury region after ice compression at different time points, and analyze the factors of the results, make relative conclusions.Methods:30 male Wistar rats are randomly divided into 4 experimental groups and a control group, the rat number of every group is 6. Use Allen's contusive model to produce spinal cord injury in that its mechanism is similar to clinical injury. Ice compression is applied to the surface of regional injury respectively from 1h,6h,8h and 12h after injury by 15g ice pack. The period of ice compression is 20minetes. Rats were sacrificed at 48h after spinal cord injury. Measure wet and dry weight of 10mm range of the spinal cord injury.Then calculate the water content of spinal cord tissue, representing edema.30 male Wistar rats are randomly divided into 4 experimental groups and a control group, the rat number of every group is 6.Use the same way to make contusive model and the same way to intervention. But the tissues are used to HE staining, Cresyl Violet Acetate staining, TUNEL staining. View specimens under 400 times microscope and count the number of apoptotic cells of randomized 5 light areas, representing the degree of spinal cord apoptotic cells. Use variance test and further statistical description and analysis to deal with the result.Results:The intervention groups and control group have no differences of the regional edema in spinal cord injury; The groups 6H,8H,12H compared with the control group have different numbers of apoptotic cells.Group 1H compared with the control group has no difference in the number of apoptotic cells. The number of apoptotic cells among 6h,8h,12h groups has no differences. That shows ice compression can effectively reduce the number of apoptotic cells, but the edema is no difference among the groups, probably due to the spinal segment of measurement, and measuring methods, intervention time.Conclusions:1. Ice can reduce the number of apoptotic cells in regional area of spinal cord injury, it is beneficial for spinal cord injury in neuronal protection. There is no statistical difference in apoptotic cell number among Groups 6H,8H,12H.2. There is no statistical difference among the groups in spinal cord edema level. Experimental design used in this study can not prove that ice compression on the injury of spinal cord can reduce the regional edema.