Effects Of Lycium Barbarum Polysaccharides (LBP) On Mice's Partly Immune Index In Heavy Load Exercise Condition

Objective:Study the effects of LBP on part immune index such as spleen/body and thymus/body weight ratio,T-lymphocyte subsets,IgG of high swimming exercise in mice.we not only discuss the relationship between heavy load of sports training and the declining of immune function,but also explore the protection mechanism for rats'exercise-induced immune suppression by LBP.Method:Seven-week-old male Kunming mice with 36,were took a swimming test,eliminate 6 mice for drowning or swimming ability deficiencies,the rest of the 30 only randomly divided into 3 groups:quiet control group(C),simple sports group(S) and sports perfusion group(SP),and each group has 10.C group took NS 0.1mL/10(g·d) during the each day from the third week,but without sports;S and SP two groups were adaptively fed for one week,adaptively trained for one week,then perfused 6 weeks,S group took NS 0.1mL/10(g·d) and SP group took LBP 700 mg/(kg·d) during the each day for mice.Three groups'index on immunity to be tested after 8 weeks:spleen/body and thymus/body weight ratio,CD4+%,CD8+% and CD4+/CD8+,IgG,each group carries on the contrastive analysis.Using McAb indirect immunizing fluorescence way to detect the T-lymphocyte subsets,using convection immune electrophoresis experiment way to detect IgG.Results:(1)Compared with the quiet control group,the thymus/body weight ratio of the simple sports group was significantly declined(P<0.05),the spleen/body weight ratio was declined too,but not evidently(P>0.05).The thymus/body and spleen/body weight ratio of the sports perfusion group were not varied evidently(P>0.05). (2)CD4+% and CD4+/CD8+ ratio of the simple sports group were very lower evidently than the quiet control group(P<0.01),CD8+% was declined too,but not evidently(P>0.05).CD4+% and CD4+/CD8+ ratio of the sports perfusion group were evidently higher than the quiet control group(P<0.05),CD8+% was changed but not evidently(P>0.05).(3)Compared with the quiet control group,IgG declined evidently in the quiet control group(P<0.05). IgG of the sports perfusion group was increased slightly,the difference has no statistically significant(P>0.05).Conclusion:The heavy load exercise can decrease the weight of immune organ,T-lymphocyte subset CD4+ and the ratio of CD4+/CD8+,IgG.To supplement the LBP can protect the injure of the immune organ,prevent the die of the T lymphocytes and the decline of the IgG.