Bone Marrow-Derived Stem Cells Contribute Skin Regeneration In Skin And Soft Tissue Expansion

Objective Skin and soft tissue expansion stimulates the proliferation of skin epidermal basal cells and increase the dermal collagen deposition and angiogenesis. To explore the contribution of bone marrow-derived stem cells (BMSCs) to the generation of "new" skin during the expansion, we used a chimeric mouse model in which the donor C57BL mice were engrafted with the bone marrow of enhanced green fluorescent protein (EGFP) transgenic mice.Methods Bone marrow-derived stem cells were collected from the tibia and femur of EGFP+ transgenic mice, and then injected into normal C57BL mice via the tail vein (Chimeric Mice). The Chimeric mice were randomized into groups A, B and C. Silicone expanders were implanted in the backs of the Group A mice and then were injected with normal saline (N.S.); silicone expanders were also implanted in the backs of the Group B mice but the expanders were not injected with N.S.; and no expanders were implanted in the Group C mice. Skin was obtained at different times (day 0,7,14,21,28 and 35). Skin Stromal-Derived Factor-1(SDF-1) expression was evaluated. The number, distribution and phenotype changes of EGFP+ cells in the skin were also evaluated by means of fluorescent microscopy.Results EGFP+ cells were present stably in the normal skin. The number of EGFP+ cells of the Group A mice changed with the tension, and reached the peak on day 21(17.1±6.7%), as compared with either Group B (5.5±1.0%) or Group C(5.1±0.9%). The SDF-1 expression in the expanded skin was significant increased (≈11-fold, p<0.01) compared to non-expanded skin on day 21. Immunofluorescence showed EGFP+ cells were converted into vascular endothelial cells, epidermal cells and spindle-shaped dermal fibroblasts.Conclusion Strain can promote the expression of SDF-1 and facilitate the differentiation and proliferation of bone marrow-derived stem cells in the expanded skin.