Research On Processing Technology And Quality Evaluation Of Rhizoma Coptidis Steamed With Rice Wine

Coptis chinensis Franch, first recorded in Shen Nong's Herbal, has the function of clearing hear, depriving dampness, and detoxicating. According to the needs of TCM diagnosis and treatment, it has processed with rice wine, ginger juice, evodia rutaecarpa decoction, vinegar and other processing methods. Since the the book of Ming yi bie lu,the Rhizoma Coptidis steamed with rice wine have superiority advantages in the treatment of diabete in many medicine books. Such as Ben cao gang mu record"Treat diabete use Rhizoma Coptidis steamed with rice wine" etc.. It can be seen, Rhizoma Coptidis steamed with rice wine treat diabete, has sufficient practice basis.In preliminary studies on Rhizoma Coptidis steamed with rice wine, we find that it has preventive and therapeutic effect on diabetes B. In this paper we use Rhizoma Coptidis steamed with rice wine with the therapeutic action on diabete as breakthrough point, We determine multicomponent contents in Rhizoma Coptidis steamed with rice wine, establish fingerprint of Rhizoma Coptidis steamed with rice wine, determine the biological activity in Rhizoma Coptidis steamed with rice wine, Analysis correlation between them. Aim to do macro research on Active ingredient which improve Insulin resistance, processing technology and quality evaluation of Rhizoma Coptidis steamed with rice wine.Objective:Explore active ingredients which improve Insulin resistance of Rhizoma Coptidis steamed with rice wine, select its best processing technology, establish its quality control methods.Method:1.Establish fingerprint of processed products of Rhizoma Coptidis, determination method for six alkaloids in processed products of Rhizoma Coptidise, serum fingerprint of Rhizoma Coptidis steamed with rice wine by HPLC.2.Use IR model glucose utilization as indicator, select its processing technology. Analyze relativity between the fingerprint and bioeffect by statistical method of multiple regression, refer components into the serum of serum fingerprint, be sure the active ingredient of Rhizoma Coptidis steamed with rice wine. Use total content of three alkaloids which related IR as indicato, select the best processing technology of Rhizoma Coptidis steamed with rice wine.3.Based on Determination of Rhizoma Coptidis in Chinese Pharmacopoeia edited in 2010, determine six alkaloids in Rhizoma Coptidis steamed with rice wine, establish its TLC method, complete its Check items (moisture content, ash content, extractive content. Establish its quality control method.Result:1.Fingerprint of processed products of Rhizoma Coptidis was established. 12.chromatographic peaks was demarcated:7. jatrorrhizine,8. columbamine,9. epiberberine,10. coptisine,11. palmatine,12. berberine. they have a good similarity by Similarity analysis and angle cosine analysis, but, they are different by euclidean distance analysis, Rhizoma Coptidis steamed with rice wine was classed together by principal component projection points analysis. The determination method for six alkaloids was established, The six alkaloids have a good linear relationship (r>0.999) and recoveries(95%-105,RSD<2%). Rhizoma Coptidis steamed with rice wine was classed together by Principal component projection points analysis, it has the highest Principal component score 0.9412, Columbamine, epiberberine and berberine are the different components in processed products of Rhizoma Coptidis by variance analysis. Serum fingerprint of Rhizoma Coptidis steamed with rice wine was established, Columbamine, epiberberine, palmatine, and, berberine were the into blood components.2.Use IR model glucose utilization as indicator, processing technologies are rice wine 20%, moistened 2h, steamed 8 h and rice wine 60%, moistened 2h, steamed 8 h. Jatrorrhizine, columbamine, and, berberine related to IR model glucose utilization. Use total content of three alkaloids which related IR as indicator, processing technology is rice wine 20%, moistened 2h, steamed 8 h.3.The quality Standard of Rhizoma Coptidis steamed with rice wine was Initially established.Conclusion:1.The fingerprint and determination method for six alkaloids established by HPLC can be used to control the quality of processed products of Rhizoma Coptidis, the contentsof Chemical composition in processed products of Rhizoma Coptidis are different, the different composition may be columbamine, epiberberine and berberine. Serum fingerprint established by HPLC can be used to study Rhizoma Coptidis steamed with rice wine, Columbamine, epiberberine, palmatine, and, berberine were the into blood components.2.The processing technology of Rhizoma Coptidis steamed with rice wine was selected, The quality Standard Initially established can be used for the quality Control of Rhizoma Coptidis steamed with rice wine, provide reference and basis for quality Control and clinical application of Rhizoma Coptidis steamed with rice wine.