A Research Of Lung Excessive Heat Syndrome Animal Model Based On Traditional Chinese Medicine Theory

According to classical Chinese medicine theory-"heat transformed from wind-cold", simulated a kind of "Lung Excessive Heat Syndrome" animal models, which consistent with the consistent theory of traditional Chinese medicine pathogenesis, and can be used in clinical or experimental study. The model group rats were stimulated by cold wind 4 days, then driped lipopolysaccharide into intratracheal to simulate and accelerate heat from the cold syndrome to permit the transformation process, to establish the Lung Excessive Heat Syndrome animal model. Collected rats' lungs, spleen and blood samples before the experiment,the fourth day and after the mole settleing.To observed the tats behavior and symptoms,lung tissue pathology, blood changes, MPO expression level and IL-2 levels of lung tissue homogenate, NK cell activity of spleen cell suspension. Observation Compared the differences between model rats and normal rats of each index, and compared Cold Syndrome and Heat Syndrome model of above-mentioned indexs, to further explore the essential difference between the two syndromes.The results show that, the animal model under the guidance of the classical theory of TCM is successful and scientific. It not only consistented with clinical manifestations of Lung Excessive Heat Syndrome, but also with the natural incidence and transmission process of the syndrome, and more suitable for Chinese medicine clinical and experimental studies copmpered to animal models which were adopted by a single stimulate factor such as viruses or bacteria before. The indexes in the experimental testing has shown a dynamic change trend, the comparison of cold syndromes and heat syndromes is also quite different before and after the modle settling.