Expression And Implication Of C-Met And OPN In Cervical Squamous Cancer

Background and ObjectiveCervical cancer is the most common cancer from female reproductive system. In our country its incidence and mortality have been increasing year by year. Cervical cancer seriously endangers women's health and the cervical squamous cancer is the most common in cervical cancer. The invasion and metastasis of carcinoma is the main reason for the death of patients with cervical cancer. Invasion and metastasis of cervical cancer is a complex and multi-step process. But the exact mechanism is not clear. Recently, invasion and metastasis has been the focus on the research of cervica cancer.The transmembrane tyrosine kinase c-Met is the receptor for hepatocyte gorwth factor(HGF). C-Met activated by its ligand leads to a variety of cellular effects, including the induction of proliferation and cell motility under both normal and pathological conditions. Likewise, HGF/c-Met play an important role in the development of tumors. OPN is a phosphorylated glycoprotein containing an arginine-glycine-aspartic acid (RGD) cell adhesion sequence and has many significant functions. But high expression of Osteopontin(OPN) is related to cancer metastasis. Through its specific receptor integrin and CD44, OPN can enhance proliferation, migration and invasion of tumor cells.Today, there aren't any reports on c-Met correlation with Osteopontin in cervical squamous cancer in the literature. This study aims to investigate the expression of c-Met and Osteopontin in cervical squamous cancer and their roles in carcinogenesis, invasion and metastasis of cervical cancer.Material and Methods1 Experimental group50 cases of sugrically resected cervical squamous cancer tissues from the Second Affiliated Hospital of Zhengzhou University from March,2007 to September, 2009 were treated as the researching group12 cases of normal cervical tissues and 36 cases of cervical intraepithelial neoplasia(CIN)tissues were treated as the control group.2 Experimental methodsStreptavidin perorxidase immunostaining technique was used to examine the expression of c-Met, OPN in normal cervical tissues, cervical intraepithelial neoplasia(CIN)tissues and cervical squamous cancer tissues.3 Statistics analysisAll of the statistical analysis was performed with statistical package SPSS version 16.0 software. Association between parameters was assessed by chi-square test or spearman stage relate analysis. a=0.05 was considered as statistically significant value.Results1 c-Met's expressions in different cervical tissuesThe positive expression rate of c-Met in cervical squamous cancer was significantly higher than that in normal cervical tissues or cervical intraepithelial neoplasia(CIN)tissues (P<0.01). There was no statistically distinct difference in c-Met expression between normal cervical tissues and CIN tissues (P>0.05).The positive expression rate of c-Met was no statistically distinct difference among clinicalstageâ… a,â… b, andâ…¡a period (P>0.05). Likewise, there was no statistically distinct difference with c-Met expression in terms of histological grading (P>0.05). However, to compare the positive rate with lymph node metastases cases and without lymph node metastases cases, a statistically distinct difference in c-Met expression was found(P<0.05).2 OPN's expressions in different cervical tissuesThe positive expression rate of OPN in cervical squamous cancer and was significantly higher than that in normal cervical tissues or CIN tissues (P<0.01). There was no statistically distinct difference in OPN expression between normal cervical and CIN tissues (P>0.05).The positive expression rate of OPN was no statistically distinct difference among clinicalstageâ… a,â… b, andâ…¡a period (P>0.05). Similarly, there was no statistically distinct difference with OPN expression in terms of histological grading (P>0.05).And yet, to compare the positive rate with lymph node metastases cases and without lymph node metastases cases, we find a statistically distinct difference in OPN expression.(P<0.05).3 The correlation between c-Met and OPN in cervical cancerThere was a closely positive correlation between OPN and c-Met in cervical squamous cancer. (r=0.600, P<0.01).Conclusions1 c-Met and OPN might play important roles in the occurrence and development of cervical squamous cancer.2 The expression of c-Met and OPN might be both involved in the metastasis of cervical squamous cancer.3 c-Met is significantly positively correlated with osteopontin in the occurrence, development and metastasis of cervical squamous cancer.4 c-Met and OPN might become biomarkers of prognosis of cervical squamous cancer and provide intervention target for gene therapy.