Electrophysiological Analysis Of The 5-HT Neurons In The Dorsal Raphe Nucleus After Stimulation In The Substantial Nigra Pars Compacta In Rats

Objective:Clinically, dopamine and 5-HT nervous system, the imbalance of the nervous system will result in steady-state many serious diseases, and even endanger the lives of patients, causing irreparable damage.Currently,the linkage of dopamine neurons and 5-HT neurons in the central nervous system are not clear. Dense central area of dopamine neurons in substantia nigra and central 5-HT neurons in the dorsal raphe nucleus intensive area has become a research hotspot. Research has shown that the substantia nigra pars compacta and dorsal raphe nuclei in the nerve fiber links. Since 5-HT neurons in a variety of subtypes of its role in dopamine neurons complex, but to suppress the main.At the same time, the study found that the 5-HT neuronal activity in dorsal raphe nucleus has changed significantly in the Parkinson's rat model.To identify the electrophysiology between the substantial nigra pars compacta(SNpc) and dorsal raphe nucleus(DRN).Methods:Using the methods of electrical stimulation and microinjection and extracellular discharge recording to observe the discharge of the 5-HT neurons in DRN.Results:First,of responsive neurons to SNpc stimulated,72% 5-HT neurons in the DRN were suppressed Second,of responsive neurons to SNpc blocked,70% 5-HT neurons in the DRN were activated.Conclusion:Alterations in the monoamines dopamine (DA) in the SNpc can affent the firing rate of 5-HT neurons in the dorsal raphe nucle-us. DA neurons in the SNpc could potentially inhibit the function of 5-HT neurons in the DRN via actions on the Subthalamic nucleus, entope- duncular.And Some nerve fibers(e.g:GABAergic) were also involved.