Effects Of The Hepatocyle Growth Factor And Calcitonin Gene Related Peptide On Fushion Of Childhood Inguinal Hernia In Vitro And In Vivo

Objective: To investigate the effects of hepatocyte growth factor(HGF) and calcitonin generelated peptide(CGRP) on childhood inguinal hernia.Methods: Levels of serum HGF and CGRP were measured by ELISA in 60 male childrenwith the inguinal hernia (experimental group 1)and 30 male healthy children (control group 1);The densities of HGF receptor and CGRP receptor on 60 hernia sacs ( experimental group 1) and30 peritoneums (control group 2, patients with non-processus vaginalis disease but open surgery)were measured by flow cytometry (FCM) technique；HGF levels were measured by ELISA in 15non–CGRP-treated cultures medium assays and CGRP-treated cultures medium assays(experime-nt group 2, 15 male children with the inguinal hernia).Results:1. Change of HGF and CGRP in serum in childhood inguinal hernia: (1) There was nosignificant difference in control group 1 which were divided into three different age grades(P＞0.05). (2) Levels of serum HGF and CGRP in the experimental group 1 were significantly lowerthan those in the control group 1(P＜0.05), among which, levels of serum HGF and CGRP werethe lowest in 6 months≤age＜1 year group (P＜0.05) , but there was no significant difference inother morbid groups(P＞0.05).2. The densities of HGF receptor and CGRP receptor in the experimental group 1 andcontrol group 2 : (1) The densities of HGF receptor on hernia sacs and peritoneums:①Therewere the lowest densities or no HGF receptor on the peritoneum mesenchymal cells (5.21±2.18),but there were more larger densities on the hernia sacs mesenchymal cells (52.23±53.48) (P＜0.05).②There was no significant difference in the density of HGF receptor on hernia sacs andperitoneums mesothelial cells (P>0.05).③The densities of HGF receptor on the peritoneummesenchymal cells (5.21±2.18) were significantly lower than on the peritoneum mesothelialcells (20.77±11.13) (P＜0.05), but the densities of HGF receptor on the hernia sacs mesothelialcells (29.44±20.36) were lower than on the hernia sacs mesenchymal cells (52.23±53.48) (P＜0.05). (2) The densities of CGRP receptor on hernia sacs and peritoneums:①The densities ofCGRP receptor on the peritoneum mesenchymal cells (69.78±51.43) were lower than on thehernia sacs mesenchymal cells (106.47±106.17) (P＜0.05); among which, the densities ofCGRP receptor were the largest in 1 year≤age＜3 years group (169.32±137.08) (P＜0.05) , butthere was no significant difference in other morbid groups (P＞0.05).②There were the lowestdensities or no CGRP receptor on the hernia sacs mesothelial cells (6.16±6.51), but there weremore larger densities on the peritoneum mesothelial cells (22.73±15.45) (P＜0.05).③The densities of CGRP receptor on the peritoneum mesothelial cells (22.73±15.45) were lower thanon the peritoneum mesenchymal cells (69.78±51.43) (P＜0.05), but the densities of CGRPreceptor on the hernia sacs mesothelial cells (6.16±6.51) were significantly lower than on thehernia sacs mesenchymal cells (106.47±106.17) (P＜0.01). (3) There was no significantdifference in the densities of HGF receptor and CGRP receptor on the unilateral and contralateralhernia sacs (P＞0.05).3. Change of HGF in cultures medium in childhood inguinal hernia: (1) Culture medium obt-aine from hernial sacs in experimental culture at 0, 24, and 48 hours was assayed for human HGF content by ELISA, HGF levels increased over time in both cultures treated with CGRP and withoutCGRP. (2) Experimental culture at 24 hours, there was no significant difference in the levelof HGF in both cultures treated with CGRP and without CGRP (P＞0.05); experimental cultureat 48 hours , there were difference in the level of HGF between cultures treated with CGRP andwithout CGRP (P＜0.05); HGF levels in cultures treated without CGRP were lower than in cultu-res treated with CGRP in 6 months≤age＜1 year group in 2 of 15 (24h: P＜0.01,48h: P＜0.01)Conclusion:1. Effecting of the HGF and CGRP on fushion of childhood inguinal hernia, It may be acritical period in 6 months≤age＜1 year and 1 year≤age＜3 years.2. Exogenous CGRP may be responsible for HGF elevation and potentially implicates defici-ent endogenous CGRP as one cause for inguinal hernia patency.3. Effects of HGF and CGRP on the closure of processus vaginalis are diversity, and theclosure of regulation process related to HGF and CGRP are complex.