The Change Of Exhausted Exercise On The Expression Of NF-kappaB, BDNF In Hippocampus Nucleus In Rats

Objective:To observe the expression of NF-κB, BDNF in different times after 6 week exhausted swimming exercise . At the same time to investigate the mechanism of the injury and repair after exhausted swimming exercise in central nervous system .Methods: seventy healthy SD rats were randomly divided into control group(n=10) and exhausted swimming group(n=60).The fresh cerebral tissues were divided on icewater bath while the paraform-perfused brains were obtained at 0 h, 4 h,8 h,12 h, 16 h, 24 h after six-weeks exhausted exercise, the former to investigate the changes of NF-κB, BDNF in amygdaloid nucleus with western-blot method, the latter to observe the expression of NF-κB and BDNF by immunohistochemical SABC techniques.All of the experimental data were analyzed by SPSS13.0 statistical software, and expressed by (Mean±S.E.).Difference was considered statistically significant at P <0.05.Results:(1)Western-Blot results:compared with control group,the expression of NF-κB in hippocampus nucleus increased at 4 h(P <0.05),and peaked at 8 h(p<0.05), decreased at 12-16 h(p<0.05),and returned to normal levels at 24 h(P >0.05). The expression of BDNF at 4h,6h,12h,16h,24h were significant higher than the control group and 0h group(P<0.05). (2)Immunohistochemistry results: the expression of NF-κB was extremely lower and mainly resided in endochylema in control group and 0 h group,no difference was found between them. Increased and transfered into nucleus at 4 h after exhausted exercise(P< 0.05), and reached peak levels at 8 h(P< 0.05),and decreased at 12 h(P< 0.05),16 h(P< 0.05),24 h(P> 0.05) gradually.Compared with control group,the number of BDNF positive cells increased in all exercise groups dramatically(P< 0.05),exclude 12 h group reached peak levels (P< 0.05),and significant between other groups(P> 0.05).Conclusion: (1)The NF-κB signaling pathway can be activated in hippocampus nucleus in rats by exhausted swimming exercise. It is closely correlated with the inflammatory reaction and apoptosis during exhausted exercise.(2)Exhausted exercise could up-regulate the expression of BDNF,which might play an important role in protecting the neurons of rat hippocampus.