Study On The Changes And Significance Of The CD4~+ CD25~+ CD127~- And CD3~+ CD4~- CD8~- Treg Cells Of T Lymphocyte In Children With Idiopathic Thrombocytopenic Purpura

Objective: To study on the changes and significance of the CD4~+CD25~+CD127~-and CD3~+CD4~-CD8~- treg cells in children with ITP.Materials and methods: 1. experimental object: 33 children cases with ITP and 21 children cases with the controls, each one was drawn 1 ml peripheral blood, anticoagulated. 2. Index detection:①Staining:1ml fresh peripheral blood anticoa -gulanted by EDTA of children with ITP and controls, test tube A is appended with three labeled monoclonal antibody (CD4~-FITC/CD8~-PE/CD3-PC5)10μl, test tube B is appended with CD4~-PC5,CD25 -FITC,CD127~-PE monoclonal antibody each 5μl,then each test tube appended with 20μl anicoagulated complete blood, mixed, away from light 30 minutes.②Hemolysis:Each test tube is appended with 100μl hemolysin, mixed, ecaded from light 15 minutes,then centrifuged for 5 minutes, by 1200 r/min, deserting the upper liquid.③Ablution: Putting NS 1 ml in above-mentioned residue liquid, mixed,centrifug;ed(ditto), deserting the upper liquid.④Awaiting to be detected: Putting NS 0.5ml in abovementioned residue liquid, awaiting to be detected.We choose 10000 cells from each semple,and detecting the expression of CD4~+CD25~+CD127~-and CD3~+CD4~-CD8~- by flow cytometry CELLQUGST software.3.Statistical assay, the data was dealt by the SPSS 11.0 statistical software, experimental data was indicated by mean±standard deviation ( x±s,%),a =0.05 is the size of test.And the analysis of variance is used.Results The expression levels of CD4~+CD25~+CD127~-and CD3~+CD4~-CD8~- treg cells were significantly lower than those of NC,which were (2.65±1.68)%VS (4.80±1.55)%(P<0.01),(5.15±3.13)%VS(8.07±3.56)%(P<0.01).Conclusion CD4~+CD25~+CD127~-and CD3~+CD4~-CD8~- treg cells may play a role in the immunologic PathoPhysiology of ITP.