Effects Of Intermedin Pretreatment To Repairing Genes Of Rat With Injured Kidney By Ischemia-reperfusion

Objective To investigate effects of Intermedin (IMD) pretreatment to repairing genes such as Vimentin of the rats with injured kidneys by ischemia reperfusion injury (IRI) during the repair and regeneration process.Method A total of 144 healthy male Wistar rats were randomly divided into 4 gourps:control group,IRI group,empty plasmid group (EP)and IMD group. After resection of right kidneys of the rats, plasmid was transfected into the left kidneys by using ultrasonic microbubble technology. After one week, we programmed the renal IRI models. We kept the samples of renal tissues after 1d, 2d, 3d, 4d, 7d and14d of reperfusion respectively, then we detected the changes of mRNA expression of the Pax-2, ZO-1, Ncam, Wt-1 and Vimentin of renal tissues by RT-PCR; analysised the changes of protein expression of Pax-2, Wt-1 and Vimentin by Western blotting and measured the changes of protein expression of ZO-1 and Ncam by ELISIA.Results (1) Compared with the expression of protein in control group, the expression of Pax-2, Zo-1, Ncam, Wt-1 and Vimentin of the rats in IRI group increased significantly in 1d to 3d(P<0.01). After 4d, the kidney of the rats in IRI group returned to the normal level (P> 0.05). (2) In IMD group, the expression of Pax-2, Zo-1, Ncam, Wt-1 and Vimentin were significantly higher than that in the other 3 groups (P <0.01) at the same time after IRI 1d to 4d. (3) It has no statistic meaning to compare the genes mentioned above in IRI group, EP group, IMD group with control group respectively(P> 0.05). It has no statistic meaning either to analysis the different expression between IRI group and EP group, though the expressions of the genes in IRI group and EP group increased compared with control group after 4d. (4) It has no statistic meaning to analysis the difference between IRI group and EP group (P> 0.05). (5) Changes of mRNA expression of genes mentioned above by RT-PCR are consistent with changes of protein expression by Western blotting or ELISIA.Conclusions IMD pretreatment plays an important role in increasing the expression of repair related genes such as Pax-2, Zo-1, Ncam, Wt-1 and Vimentin during the process of repair and regeneration after renal ischemia reperfusion injury.