Study On Constituents From Propolis And Honey

In this paper, one pecie of propolis and five type of honey which were produced from Zhumadian district of Henan were studied, including total flavonoids, total phenolic acids and volatile components. The concentration of lead in propolis were also determined. Flavonoids were analyzed by aluminum chloride colorimetric method and the total phenolic content was test through Folin-Ciocalteu colorimetric method. The volatile compounds were determined by gas chromatography-mass spectrometry (GC-MS) method. The determination of lead in propolis by ultraviolet spectrophotometry is proposed with a low- cost, ,environment friend and good feasibility. The main contents can introduce detailed as follows:â‘´the determination of volatile components,Total flavonoids,Total penolics and the determination of lead in propolisThe volatile components in propolis were analysised by GC-MS, on one hand Liquid–liquid extraction and solid phase extraction(SPE) by self-made activated carbon-based SPE column were used to prepare the samples. The results show that the esters obtained in volatile components are larger by ethyl acetate extraction and SPE. the alcohols obtained in volatile components are larger by methylene dichloride extraction and acetonitrile extraction.The content of total flavonoids in propolis was determined by AlCl₃ photometry at the absorption 440nm. The linear regression equation was Y=0.01465+0.03603 X (r = 0.9994). The average total flavonoid content in propolis is 3.753%. Total phenolic content in propolis was also detected by Folin-Ciocalteu colorimetry method. The calibration equation is Y = 0.00982 +0.06965 X (r = 0.9992).And the total phenolic content in propolis is 7.524%.A new dual-wavelength peaks method for the determination of lead in water phase reaction system at pH8.0-9.0 by using dithizone - ethanol-Tween80 as the color reagent was developed. In this method, selecting 1.50 mL color dosage, pH (8.0-9.0). The calibration equation is Y=0.1778X+0.002 (r=0.9996).The lead content in propolis is 3.571 mg·kg^-1.⑵The volatile components, Total flavonoids,Total penolics in five types of HoneyThe volatile components in the five types of Honey was measured with the same SPE columns as in propolis. Comparativly analysis of different volatile components in five types of honey showed that among the Jing nectar honey, nectar Prunella honey, jujube flower Honey, Acacia honey and rape honey, isopropyl acetate only does not exist in the Jing nectar. Benzylphenol, isoamylalcohol, butyl acetate, isobutyl acetate only do not exist in the Jujube flower honey. Metoxybenzene ethanol was not detected in Acacia honey. 9-twentythree carbene, Cyclopentadecanolide were detected only in the rape honey, but phenylethyl acetate does not exist in the rape honey. octacosane and menthol were detected only in the nectar prunella honey honey. 3-methylcyclopentadienyldiol, 3-methyl-1, 3, 5-triol do not exist in Sophorae honey. All the eight components: acetate ethyl, ethyl propionate, furfural, benzaldehyde, dihydroxyacetophenone, phenylethylalcohol, dibutylphthalate, ethylhexyl phthalate all contain in the five types of honey.Total flavonoids and total phenolic content in honey are also detected by by AlCl₃ photometry and Folin-Ciocalteu colorimetry method. The results showed that the flavonoid content in Acacia honey and jujube flower honey is higher than the other three. The average total flavonoid contents in nectar prunella honey is 0.001375%,in jujube flower honey is 0.001521%,. And the total phenolic content in nectar prunella honey is 0.06328%, in isjujube flower honey is 0.05684%, in acacia honey is 0.05565%, in Jing nectar honey is 0.05531%, in rape honey is 0.05065%.