Studies On The Pharmacokinetics Of Marbofloxacin In Rats

Marbofloxacin as a third-generation fluoroquinolone antibiotics, primarily by inhibiting bacterial DNA helicase, interferenced with bacterial DNA synthesis during stages of replication and transcription, resulting in the death of bacterial cells, which play the efficacy of anti-bacterial and anti-bacterial Broad spectrum antibacterial activity and other characteristics. Therefore, in recent years it has become a focus researches in veterinary antibiotic. The analysis approach for Marbofloxacin and its pharmacokinetic study by high performance liquid chromatography and capillary electrophoresis has been established.Methods of High-Performance Liquid Chromatography and High-Performance Capillary Electrophoresis were established for the detection of Marbofloxican in this paper. In the HPLC method C18 column was used with the mobile phase of 0.02mol/L ammonium acetonitrile (73:17) at the detection wavelength of 295nm, at the flow rate of 1.OmL/min. The calibration curve of marbofloxican was linear in the concentration range of 10μg/mL-100μg/mL(r=0.9998), the recovery was 99.35%.The limit of detection was 0.025μg/mL and the content of Marbofloxican is 99.94%.In the HPCE method,15mmol/L tetraborate decahydrate buffer (pH=9.2) was used, the separation voltage was 20kv,and the injection volume of sample was 10s. The calibration curve of Marbofloxican was linear in the concentration range of 10μg/mL-100μg/mL(r=0.9993), the recovery was 100.2%.The limit of detection was 2.5μg/mL and the content of Marbofloxican is 99.92%.HPLC was higher precision and well repeated compared with the HPCE,while the HPCE was simple,economical and quickly.SD rats were treated by oral gavage administration of MBF(2.5mg/kg),while the blood samples were collected from rump within 12 hour post giving drug. The concentrations of MBF in serum were determined by high performance liquid chromatography(HPLC). The results show that Marbofloxacin in SD rats is coincidence two-compartment open pharmacokinetic model. The concentration-time equations are: C=27.37e-2.0361t+4.35e-0.0247t. The primary pharmacokinetic parameters of MBF are: t1/2α=0.69±0.02 h, t1/2β=7.63±0.40 h, AUC=37.24±0.02μg-mL-1·h, Vd=1.13±0.01 L-kg-1. It will be seen that the distribution of MBF in vivo is rapid and abroad, and the elimination of MBF in vivo is slow.SD rats were treated with a single dosage of MBF(2.5 mg/kg)intramuscularly and rapidly, while the blood samples were collected from rump within 12 hour post giving drug. The concentrations of MBF in serum were determined by high performance liquid chromatography(HPLC). The results show that Marbofloxacin in SD rats is coincidence two-compartment open pharmacokinetic model The concentration-time equations are: C=9.28e-0.3841t+4.63e-0.1006t -12.32e-0.1015t. The primary pharmacokinetic parameters of MBF are: t1/2α=0.49±0.11h, t1/2p=6.59±0.31h, AUC=42.27±3.60μg·mL-1·h, Vd=2.20±0.17L·kg-1.It will be seen that the distribution of MBF in vivo is abroad, and the elimination of MBF in vivo is slowly.