Mechanisms About The Compatibility Between Radix Polygalae And Magnolia Which Relievs The Gastrointestinal Motility Inhibition And Gastric Mucosal Injury Caused By Radix Polygalae

Objective:To explore the possible biological mechanisms about the compatibility between Radix Polygalae and Magnolia which turned out to have some kind of relieving effects in the gastrointestinal motility inhibition and gastric mucosal injury caused by Radix Polygalae.Methods:①Determinate the AchE activity and NO concentrations in rat's gastrointestinal tissue by the Use of methods Colorimetry and Nitroreductase chromatometry respectively.②Observing the VIP positive immunohistochemical products in rat's antrum③Observing the gastric mucosal injury of mice by stereomicroscope④Determinate the SOD activity and MDA concentrations in mice's gastric tissue by the methods of Hydroxylamine and TBA respectively.⑤Determinate the Na+K+-ATPase and Ca2+-ATPase activity in mice's gastric tissue by the colorimetric determination of phosphorus.Results:①The experience of determination of AchE activity shows that: compared with saline, each decoction has little effect on the AchE activity of the rat's antrum (P>0.05); while, when we determinate the AchE activity of the rat's duodenal, we found, compared with saline, Radix Polygalae has a very significant, effect on decreasing the AchE activity(P<0.01), though Magnolia impact on AchE activity so little, the compatibility between Radix Polygalae and Magnolia turned out to have a very clear effect on it,which shows that the compatibility between Radix Polygalae and Magnolia at the ratio 1:1,1:2 both can elevate the AchE activity compared with Radix Polygalae and the latter has no difference with saline(P>0.05), the compatibility between Radix Polygalae and Magnolia at the ratiol:3 can decrease the AchE activity more than Radix Polygalae(P<0.05).②The experience of determination of NO concentrations shows that:compared with saline, Radix Polygalae can increase the NO concentrations significantly of the rat's antrum (P< 0.05), Magnolia shows little effect on that, while, on the other hand, the compatibility between Radix Polygalae and Magnolia at the radio 1:1,1:2,1:3 all have a clear effect on the decrease of the NO concentrations in rat's antrum compared with Radix Polygalae (P<0.05); All the decoction has no effect on the NO concentrations in rat's duodenal.③The immunohistochemistry of VIP shows that: Radix Polygalae increased the positive immunotochemical products of VIP significantly compared with saline (P<0.01),while, Magnolia, the compatibility between Radix Polygalae and Magnolia at ratio of 1:1 shows little effect on the positive immunohistochemical products of VIP in muscular layer compared with saline (P>0.05).④The experience of observing the gastric mucosal injury shows that:compared with saline, Radix Polygalae injured mice's gastric mucosal badly(P<0.05), the injuries caused by the compatibility between Radix Polygalae and Magnolia at the radio 1:1,1:2 both more serious than those caused by saline and lighter than Radix Polygalae, but the four shows no significant difference(P>0.05),the injury caused by the compatibility between Radix Polygalae and Magnolia at the radio 1:3 was higher than these caused by Radix Polygalae(P>0.05),and which has a difference with the injury caused by saline(P<0.05).⑤The experience of determination of SOD activity. MDA and PGE2 concentrations shows that:compared with saline, other decoction all have little influence in the SOD activity of mice's gastric tissue(P> 0.05); Radix Polygalae caused a higher MDA concentrations in the mice's gastric tissue, but which shows no statistical difference with other decoction(P>0.05),as for PGE2 concentrations, we found that Radix Polygalae caused a lower PGE2 concentrations in mice's gastric tissue, which has no statistical difference with the concentrations caused by saline (P> 0.05),while, the interesting part of this experience is that Magnolia and the compatibility between Radix Polygalae and Magnolia at the radio 1:1 both caused a lower PGE2 concentrations even than saline(P <0.05), the compatibility between Radix Polygalae and Magnolia at the radio 1:3 elevated the PGE2 concentrations in mice's gastric tissue than Radix Polygalae(P< 0.05).⑥The experience of determination of Na+K+-ATPase and Ca2+-ATPase activity shows that:all the decoction has little influence on the Na+K+-ATPase and Ca2+-ATPase activity of the mice's gastric tissue(P>0.05).Conclusions:①Higher NO concentrations in antrum,lower AchE activity in duodenal,higher positive immunohischemical products of VIP in rat's muscular layer of antrum may related to the rat's gastrointestinal motility inhibition caused by the use of Radix Polygalae。②Magnolia shows no effect on gastrointestinal issues's AchE activity and NO concentrations and not increased the positive immunohistochemical of VIP in antrum.③Reducing the concentrations of NO in antrum,enhancing the AchE activity of duodenal and decreasing the positive immunohischemical products of VIP in rat's muscular layer of antrum may all related with the compatibility between Radix Polygalae and Magnolia at ratio of 1:1 which shows a good relieving effects in the gastrointestinal motility inhibition caused by Radix Polygalae.④Radix Polygalae can injure mice's gastric mucosal, while, the compatibility between Radix Polygalae and Magnolia at ratio of 1:1 and 1:2 can in some degree relieve this injury, both may not related with SOD,MDA,Na+K+-ATPase and Ca2+-ATPase.This study suggest that:reducing the NO concentrations and positive immunohischemical products of VIP in rat's antrum may related to the compatibility between Radix Polygalae and Magnolia at ratio of 1:1 which shows a good relieving effects in the gastrointestinal motility inhibition caused by Radix Polygalae.