Research Of Epitopes Of Autoantibodies Against EGFR, Her-2, Survivin And CyclinB1 In Sera Of Non-small Cell Lung Cancer Patients

ObjectiveAutoantibodies as new tumor markers may play an important role in the early diagnosis and evaluating the prognosis of lung cancer patients.In this study, we map the epitopes of autoantibodies against EGFR, Her-2, Survivin and CyclinB1 in sera of non-small cell lung cancer patients with peptide array and screen the epitopes with high frequency. Through matching the sequence between epitopes with high frequency and the 4 kinds of protein to discover the structure distribution of these epitopes.Method4 kinds of peptide array which covered all the peptides in the 4 peptide libraries were synthesized by a synthesizer(ASP SL).Mapped the epitopes of autoantibodies against EGFR, Her-2, Survivin and CyclinB1 in serum of 50 NSCLC patients,. Screened the epitopes which were recognized by the 4 kinds of autoantibodies with high frequency. Through matching the sequence between epitopes with high frequency and the 4 kinds of protein to DISCOVER the structure distribution of these epitopes.Result4 kinds of peptide array were synthesized by a synthesizer (ASP SL). There were no the 4 kinds of autoantibodies in the mixed-serum of healthy objectives and in the mixed-serum of pulmonary tuberculosis patients. 10 of 50 NSCLC patients were found to have EGFR autoantibodies; 9 of 50 NSCLC patients were found to have Her-2 autoantibodies; 8 of 50 NSCLC patients were found to have survivin autoantibodies; 8 of 50 NSCLC patients were found to have CyclinB1 autoantibodies. Serums of A006,A022,A094,A124 had all 4 kinds of autoantibodies against EGFR Her-2 survivin and CyclinB1.Through scanning the epitopes, we found the epitopes with high frequency of the 4 kinds of autoantibodies. Discovered the structure distribution of these epitopes with high frequency.ConclusionThrough epitope scanning , we found the epitopes with high frequency of the 4 kinds of autoantibodies. Through matching the sequence between epitopes with high frequency and the 4 kinds of protein we discover the structure distribution of these epitopes with high frequency. The discover of the epitopes with high frequency and the address of the the epitopes with high frequency will offer new clues for the further research of the 4 kinds autoantibodies.