| Objective: To compare high-frequency (frequency 100HZ, voltage of 3V, continuous wave), frequency (frequency 50HZ, voltage of 3V, continuous wave), low frequency (frequency 2HZ, voltage of 3V, continuous wave) electroacupuncture pretreatment on acute coronary artery ligation rabbit heart model of myocardial ischemic injury and effect of the protection mechanism for the clinical use and universal application of acupuncture treatment of ischemic heart disease and provide experimental evidence.Methods: According to Xu Shuyun other recommended method, by coronary artery ligation model of acute myocardial ischemia rabbits, to BL-420F biological function of experiment systemⅡECG and left ventricular pressure to 722 visible spectrophotometer serum LDH , CK, CK-MB , MDA, SOD content, and NBT staining of myocardial infarct measured were observed.Select 1.5 - 2.5kg healthy adult rabbits, with the BL-420F Biological Experimental SystemⅡECG was recorded, to remove abnormal electrocardiogram, EKG normal (male and female) were randomly divided into normal group, sham operation group, model group, high, medium and low frequency electro-acupuncture group and model group, 10 in each group.High, medium and low frequency electro-acupuncture group were given acupuncture pretreatment 7 days, bilateral acupoints selected points within the customs, daily 8 am to 10 points between the acupuncture once per EA 40min, with Hua Tuo card 0.30×40mm needle, Rabbit in the light of the relevant acupoints "experimental acupuncture" points of Standard map, independent of electrode in the palm, connected G6805-Ⅱelectro-acupunctu re device, electro-acupuncture parameters: low frequency was 2Hz, IF Section 50Hz, high-frequency group 100Hz, voltages are 3V, continuous wave. Normal group, sham operation group, model group, control group not given electric stimulation, but similar to 7 days the same time, the acupuncture group bundle, so that all animals under the same conditions for comparison. Each group after the last treatment, immediately 20% of urethane 5ml/kg ear vein injection of anesthesia, Yang-bit fixed in the platform, cut the neck, chest hair, iodine disinfectant, alcohol, iodine removal, hole towel shop sterile after surgery, animals received isolated tracheal breathing machine, separation of right carotid artery and insert the cardiac catheterization records of left ventricular pressure curve, while inserting the needle in the subcutaneous limb electrodes recorded ECG curveⅡ. Normal group were not exposed to the heart; sham operation group exposed to heart surgery, and then isolated coronary artery without ligation of L-branch; model group, EA group exposed to cardiac surgery, the isolated coronary artery and ligation of L-threading support; control group exposed to cardiac surgery isolated coronary artery cx, threading blocking blood flow after the first 5min, reperfusion 5min, repeated three times so a total of 30min, and then ligation of L-branch. Recorded in each group after coronary artery occlusion 10s, 1min, 5min, 10min, 30min, 60min, 90min, 120min, 150min, 180min time pointⅡECG changes and left ventricular pressure; the ligation of left common carotid artery during 360min blood 4ml, 4℃centrifugal separation of serum, according to kit required serum LDH, CK, CK-MB, SOD, MDA content; carotid artery blood immediately after the chest cut out the heart, after washing with ice along the coronary sulcus remove salt the great vessels and heart, left ventricle into five uniform cross-section, measured by NBT staining of myocardial infarction.Results: The model group significantly slower heart rate, T wave-the-art, compared with the normal group by statistically significant differences; model group ST segment change was not obvious; examination revealed left ventricular pressure, LVSP decreased in model group, LVEDP increased + dp / dtmax, -dp/dtmax slowed down compared with the normal group there were significant differences; levels of serum LDH, CK, CK-MB increased, MDA increased, SOD decreased compared with the normal group there were significant differences; model myocardial infarct size compared with the normal group there were significant differences.High frequency electro-acupuncture group T wave changes in amplitude and ST segment were improved, and low frequency electro-acupuncture group heart rate have improved significantly compared with model group were significantly different; high frequency electro-acupuncture group LVSP, + dp / dtmax significantly improved role model group differences were significant; IF EA group LVEDP, -dp/dtmax improved significantly, compared with model group, there were significant differences; low, medium and high frequency electro-acupuncture serum LDH, CK, CK-MB reduced, and the model group were significantly different; high frequency electro-acupuncture serum SOD increased, MDA decreased, and the model group differences were significant; low frequency electro-acupuncture serum SOD, MDA change and the model group had no significant differences; high frequency electro-acupuncture group myocardial infarct size, and model group differences were significant, Low frequency electro-acupuncture group change the scope of myocardial infarction compared with model group no significant difference.Indexes the control group and model group were significantly different.Conclusion:1,L-branch of coronary artery ligation in rabbits can be successfully replicated in acute myocardial ischemia model, the observed indicators changed significantly.2,Different frequencies of electroacupuncture preconditioning against myocardial ischemia induced by coronary artery ligation model of rabbit cardiac injury in rats.3,The protective effect of acupuncture increased SOD activity and decreased MDA content.4,The protective effect of acupuncture to improve left ventricular systolic pressure, left ventricular diastolic pressure and left ventricular pressure terminal maximal rate of the.5,Model group can serve as a means of preconditioning against myocardial ischemia, it has to be examined in depth. |
PDF Full Text Request