The Establishment Of U251 Cells Infected With HHV-6 In Vitro Model And The Preliminary Study Of HHV-6 Affecting The Biological Function Of U251 Cells And Its Mechanism

Human herpes virus-6 (Human herpesvirus 6, HHV-6) is a kind of double-stranded DNA in human lymphocytes addicted virus. In 1986, Salahuddin et al were the first to isolate HHV-6 using peripheral blood mononuclear cells obtained from patients with lymphoid hyperplasia in the American Cancer Center. Two genetically distinct variants of the virus exist, HHV-6A and -6B. HHV-6 is aβ-herpesvirus related to HHV-7 and, to a lesser extent, human cytomegalovirus (HCMV). HHV-6 infection has a broad tropism, the main violations of CD4 + T cells, monocytes macrophages and glial cells. In 1988, Yamanishi et al confirmed HHV-6 is the pathogen of acute measles in infants and children (ES). Later, HHV-6 has been linked with many other kinds of diseases, including glioma, encephalitis, chronic fatigue syndrome, post-transplant infections and multiple sclerosis,although the significance of the associations is unclear.Glioma is the most common primary brain malignant tumor. To date, the etiology is unclear.Recently, the role of virus in the development and progression of gliomas has caused widespread concern. A number of articles have reported that HHV-6 DNA can be detected by PCR in glioma tissue , but failed to get the virus. Human glioma U251 cell is a glioma cell line with some characteristics of astrocytes. So if it can be infected with HHV-6 in vitro, the study of relationship between HHV-6 and glioma can be carried.The thesis contains three parts, (i) HHV-6 GS was inoculated in CBMCs, HHV-6 DNA and antigen were detected by PCR and IF respectively. Virus titer was determined as 5×103TCID50/ml. (ii) U251 cells can be infected with HHV-6 in vitro, resulting in CPE, cell proliferation, alteration of cell cycle and increasing the secretion of IL-6. (iii) The study of JNK and P38 pathway related todvision and differentiation of tumor cells. The results showed that HHV-6 increased the expression of the phosphorylated JNK and P38 proteins in U251 cells. Maybe the virus can induce the differentiation of tumor cells through activating JNK and P38 pathway.The establishment of U251 cells infected with HHV-6 in vitro and the preliminary study of HHV-6 affecting the biological function of U251 cells and its mechanism illustrated that HHV-6 was linked to the occurrence and development of glioma,.It provides the theoretical and experimental basis to further study the etiology and pathogenesis of glioma.