| Objective To investigate the effect of Resveratrol on the proliferation and the expression of high glucose-activated transforming growth factor-β1 (TGF-β1), AMP-activated protein kinase phosphorylation (p-AMPK) in rat mesangial cells (HBZY-1). Methods (1) Cultured rat mesangial cells were exposed to D-glucose at different concentrations or different time, respectively. Normal and D-mannitol group served as control. HBZY-1 cell proliferative rate within 6, 12 and 24h were detected by MTT colorimetry. Cell total RNA and protein were extracted. The mRNA levels of TGF-β1 was measured by RT-PCR and the protein levels of TGF-β1, p-AMPK were detected by Western blotting. (2) Cultured rat mesangial cells were exposed to Resveratrol at different concentrations for 1h, and then to high glucose (30mmol/L) for 48h. The protein expression of TGF-β1, p-AMPK in rat mesangial cells were determined by Western blotting. Results (1) As compared to normal and D-mannitol control group cells, the proliferation of rat mesangial cells induced by high glucose were significantly high, the mRNA and protein levels of TGF-β1 was increased and the protein levels of p-AMPK was inhibited in cells exposed to high D-glucose in a dose and time-dependent manner. Their difference all had significance (P value less than 0.05). (2) Compared with high glucose group, resveratrol could inhibit the proliferation and the expression of TGF-β1 and increase the expression of p-AMPK of HBZY-1 cells cultured in high glucose. Their difference all had significance (P value less than 0.05). Conclusions High glucose can increase the expression of TGF-β1 and inhibit the expression of p-AMPK in HBZY-1 cells in a dose and time-dependent manner. Resveratrol can inhibit the proliferation and the expression of TGF-β1 and increase the expression of the expression of p-AMPK of HBZY-1 cells under high glucose and may play a role in the treatment of diabetic nephropathy. |
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