The Study Of The Separation Method For Colistin Sulfate And Colistimethate Sodium By Micellar Electrokinetic Capillary Chromatography

Colistin is a alkaline peptide antibiotic (polymyxins E) produced by strains of Bacillus polymyxa var. In 1960s, colistin was used for the treatment of Gram-negative bacteria infections, but because of its side effects including renal toxicity and neurotoxicity, the application of colistin was limited, and was gradually instead by others which are good efficacy, low toxicity. However, with the emergence of drug-resistant strains of Gram-negative, especially multidrug-resistant of infections and the emergence of new drug development are limited, colistin receive attention again. At home and abroad, bioassay was used to detect colistin in the most of the cases, and High Performance Liquid Chromatography (HPLC) achieved the separation of colistin using pre-column or post-column derivatization, both of them were complicated. Micellar Electrokinetic Capillary Chromatography (MECC) which is based on micellar solubilization and capillary zone electrophoresis, can be used as a great potential of the chromatographic techniques for separation of proteins, and the method is simple, rapid, practical. At present MECC which was used to separate colistin has not been reported.In chapter 1, the development of the MECC was introduced, and the principle, character and application of the MECC were also described.In chapter 2, polymyxin E1 and polymyxin E2 in polymyxin E sulfate was determined using MECC. Several factors including the running voltage, the type of surfactant, concentration of Brij35 (polyoxyethyleneglycol dodecyln ether), NaCl solution and acetonitrile, pH of phosphate were investigated.In chapter 3, colistimethate sodium in synthetic product was determined by MECC, the method of precision and reproducibility was examined.