Research Of Impact On The Survival Rate Of Correlated Clinical Factors Of Gastric Cancer Patients Treated With CIK Cell Therapy

Objective To generate cytokine-induced killer ( CIK ) cells by inducing lymphocytes cells from peripheral blood in vitro,and analyze the impact on the survival rate of correlated clinical factors of gastric cancer patients treated with cytokine-induced killer cells adoptive immunotherapy.Methods Lymphocyte separation medium was used for the separation of the peripheral blood mononuclear cells ( PBMCs ) . PBMCs were activated with IFN-γrhIL-2,rhIL-1α,anti-CD3McAb.The phenotype of CIK cells were analyzed by FACS,and the cytotoxic activity were tested by MTT.and then CIK cells after induced and cultured were infused back to gastric cancer patients. Retrospective cohort study was adopted, the median survival time and survival rate were estimated with Kaplan-Meier, and the impact on the survival rate of clinical factors were compared by Log-rank test.Results The number of CIK was increased significantly during cell culture, reaching the highest on day 14 to 21.The recurrence rate was significantly lower in CIK treatment group than that in chemotherapy group(53.3% vs 71.6%), and the difference was statistically significant(χ2=5.566,P=0.018). The median survival time(months) of gastric cancer patients in CIK treatment group was significantly longer than that in chemotherapy group(49 vs 27), and the difference was statistically significant(χ2=10.907,P=0.001). In CIK treatment group, the therapeutic efficacy and prognosis was significantly affected by tumor size, whether to relapse, pathological grade and tumor stage, and the difference was statistically significant. Conclusions CIK cell transfusion could significantly improve the survival time of gastric cancer patients, and the therapeutic efficacy and prognosis was affected by correlated clinical factors of gastric cancer patients.