| Due to trauma, tumor, inflammation, aging and other causes of human hard tissue defects, which is the oral medicine and clinical medicine need to be solved problems. The guided tissue regeneration membrane and application of the discovery has undoubtedly brought hope to solve this problem. Currently used in clinical practice of guided tissue regeneration membrane materials can be divided into①to polytetrafluoroethylene (ePTFE) as the representative of the non-degradable material;②to previous collagen,polylactic acid (PLA) as the representative of the degradation of materials.Non-degradable material made of guided tissue regeneration requires not only the second operation to remove the membrane, but also expensive; and biodegradable materials with guided tissue regeneration membrane made of the second operation to remove though not required, but there is immunogenicity, degradation time and the shielding effect of uncertainty. Therefore, in order to overcome the above shortcomings, the experimental basis for biomimetic mineralization of the theory, that is, under mild physiological conditions, self-assembly biomimetic synthesis of a new biodegradable, with a high degree of biological activity, can activate cell gene protein expression, and promote self-healing potential of the organization a new generation of guided tissue regeneration membrane. Namely, select the two kinds of sodium alginate and chitosan with high biological activity and organization of material compatibility, used as the control matrix apatite crystal growth template. The Construction of sodium alginate / chitosan new bone-guided tissue regeneration membrane, through its continuous intake of simulated body fluid (Simulated body fluid, SBF) in the calcium and other ions in human hard tissue defects in the surface of biomimetic mineralization of hydroxyapatite induced by gray Stone (hydroxyapatite, HA) crystals generated in order to look forward to addressing human hard tissue defects problems.Objective Based on biomimetic principles, to build alginate / chitosan guided tissue regeneration membranes in simulated body fluid intake of calcium and phosphorus plasma, induced hydroxyapatite crystal growth, for human hard tissue repair defects provide the basis for biomimetic mineralization of research based on .Methods Choose dish as a membrane carrier, through the freeze-dried after treatment to form honeycomb-like structure, instrument, and then immersed in simulated body fluid by 37℃constant temperature water bath incubation 7d. And then by freeze-drying treatment after forming instrument, scanning electron microscopy resultant morphology; film X diffractometer to determine the elements and nature of the resultant; Fourier transform infra red spectrometer observation of the characteristics of resultant peaks.Results1.The visual observationMineralization front of guided tissue regeneration membrane surface and the mineralization of the film surface after the comparison, in the SBF mineralization 7d after guided tissue regeneration membrane surface can be observed by the naked eye appears on the surface of the white substance, and the material generated by color and membrane itself has a more obvious difference.2. the detection of XRD on the mineralization of guided tissue regeneration membrane surfaceAfter freeze-drying treatment on the membrane surface of the white line of X-ray diffraction the material analyzed, the X-ray diffraction diagram appeared characteristic peaks of hydroxyapatite crystals. Show incubated in a simulated body fluids, sodium alginate / chitosan built into the guided tissue regeneration membrane can induce the generation apatite crystals.3. The atlas analysis on the mineralization pre-and post-mineralized guided tissue regeneration membrane surface material with FTIR spectrometerBiomimetic mineralization after 7 days of guided tissue regeneration membrane appeared on the surface of the white substance detected by Fourier transform infrared spectroscopy atlas shows the HA crystal phosphate vibration bands characteristic peaks and carbonate absorption peaks, which is without Biomineralization The maps are not available in. Visible sodium alginate / chitosan built into the guided tissue regeneration membrane surface of the white crystals of hydroxyapatite carbon acid radical.4. Observe mineralized guided tissue regeneration membrane surface materialwith scanning electron microscopyWill be guided tissue regeneration membranes in simulated body fluid for biomimetic mineralization of seven days after the scanning electron microscope, amplified 600 times and 10500 times as you can see under the conditions of the surface membrane gaps of varying sizes with irregular cracks and voids and cracks the internal structure of the mesh. In the 1500-fold and 10500-fold amplification conditions, showed the white membrane surface of the material for the cluster-like, plate, block structure, crystal show diversity.Conclusion The generation of hydroxyapatite crystals can be induced by uptaking calcium and phosphorus in SBF through constructing the sodium alginate/chitosan membrane guiding tissue regeneration. For due to trauma, tumor, inflammation, aging and other causes of human hard tissue defects provide an objective basis. |
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