Research On Differences In Serum Proteomics Among Pregnancy-induced Hypertension Patients

Objective:To search for high sensitive and specific serum markers for the early diagnosis of pregnancy-induced hypertension.Method:8 patients who were diagnosed with severe pre-eclampsia were selected randomly in First Affiliated Hospital of Anhui Medical University . The one who was diagnosed with some kinds of disease which influnced the content of serum protein was excluded from our research. Among healthy pregnant women at the same time,8 matched by age and gestational weeks were taken out to set as the normal comparison group. 4 ml venous blood was extracted from gestational women. The serum was separated and high abundant protein was extracted . In this study, we screened the difference of serum proteins between severe pre-eclampsia patients and healthy control group by two-dimensional gel electrophoresis and mass spectrometry. First we used 2-DE to separate serum proteins of the two groups, using image analysis software to identify the different protein spots . Different protein was identified by the electro-spray ionization tandem mass spectrometry (ESI-Q-TOF MS / MS) and the expression of substance P in serum of both groups was detected by the Western blot.Result:We identified eight different protein spots by 2-DE and mass spectrometry, and identified three different kinds of protein which contained thrombin, substance P and albumin. Western blot analysis showed that the expression level of substance P in serum of patients with severe pre-eclampsia was significantly lower than the level in the control group. Conclusion:Substance P can be used as serum molecular markers to diagnose pregnancy-induced hypertension.