Effect Of Dietary Fiber Complex On Intestinal Mucosal Barrier In Rats With Experimental Colitis

Inflammatory bowel disease(IBD) includes ulcerative colitis(UC)and Crohn's disease(CD) which is a chronic intestinal inflammation disease which cause and pathogenesis have not yet defined.IBD can cause intestinal mucosal barrier injury.DFC can maintain the normal form of intestinal mucosa, protect intestinal mucosal barrier, prevent bacterial translocation. This study investigated the Effect of DFC on intestinal mucosal barrier in IBD rats, to provide the basis for its clinical application.Objectives:To evaluate the effect of EN containing DFC on intestinal mucosal barrier in rats with experimental colitis, to provide the basis for its clinical application.Methods:1.96 SD rats which were made of IBD by acetic enemas. were divided into 3 groups at random(32 in each group):C(control) was given EN without DFC;T1 and T2 was given EN with DFC which containing different proportions of SDF and IDF.2. To measured concentration of D-lactate and DAO in plasma and histopathological scores of intestinal samples at the 1th 3th 5th and 7th day after the EN.3. Analysis D-lactate concentration in the plasma, DAO activity in the plasma and the score of colon injury in general morphology and histological with SPSS 13.0 statistical software,Measurement data were indicated with the mean±standard deviation.Using single-factor analysis of variance analysis the comparison between groups.If p<0.05,the difference was statistically significant.Results: 1. After the enteral nutrition,in the first day,difference of D-lactate concentration in plasma in each group was not statistically significant.In the third day,fifth day and seventh day,D-lactate concentration in plasma in group T1 and T2 were significantly lower than in group C (P<0.05),There was no significant difference between group T1 and T2.2. After the enteral nutrition,in the first day,difference of DAO activity in the plasma in each group was not statistically significant.In the third day,fifth day and seventh day,DAO activity in the plasma in group T1 and T2 were significantly lower than in group C (P<0.05),There was no significant difference between group T1 and T2.3. At different time points ascites were seen in rats' intra-abdominal of group C,there is smell in the intra-abdominal.Colon and rectal edema, swelling, expansion and adhere heavier with the surrounding tissue.After the enteral nutrition,the above-mentioned performance in group T1 and T2 reduced at different time points,compared with group C.The score of general form of colon injury was significantly lower (P<0.05).In the third day,the fifth day,the seventh day,The score of general form of colon injury in group T2 was significantly lower than in group T1.4. After the enteral nutrition,score of histological colonic damage in group T1 and group T2 was significantly lower than in group C at different time points(P<0.05).In the fifth day, seventh day.The score of histological colonic damage in group T2 was significantly lower than in group T1(P<0.05).Conclusion:Intestinal permeability of IBD rats which were given EN with DFC can be decreased, DFC has some protective effect on intestinal mucosal barrier.Different proportions of the SDF:IDF has different protective effect on the intestinal mucosa, to moderate increase the proportion of IDF be able to increase the protective effect.